Anti-mCherry purified

Anti-mCherry purified
Regulatory status
RUO
Antigen
mCherry
Clone
PAb (918)
Format
Purified
Variant
0.1 mg
11-918-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Product details
Description
Images
Isotype
Rabbit polyclonal
Specificity
The rabbit polyclonal antibody PAb (918) recognizes a red fluorescent protein tag mCherry.
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml.
Immunogen
mCherry protein from Anaplasma marginale
Concentration
1 mg/ml
Preparation
Purified from rabbit serum by affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Antigen description
The mCherry is a red fluorescent protein with excitation maximum 587 nm and emission maximum 610 nm. It has around 28 kDa, and it is being used as a fluorescent tag in expression systems.
UniProt ID X5DSL3
11-918_WB
Western blotting analysis of mCherry fluorescent protein using rabbit polyclonal PAb (918) on lysates of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs (HEK293T/17 cells transfected with YFP/GPI; negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rabbit anti-mCherry polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for mCherry protein at approximately 30 kDa.
11-918_FC_Dot-plot.tif
Flow cytometry surface staining pattern of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained using anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC).
11-918_FC_Histogram.tif
Separation of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mCherry/YFP cell suspension.
Variant
0.1 mg
11-918-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD