Exbio — Research products — Antibodies — Tags and labels — Anti-mCherry purified

Anti-mCherry purified

Regulatory status

RUO

Antigen

mCherry

Clone

PAb (918)

Format

Purified

Application

WB, FC (QC tested), ICC

Variant

0.1 mg

11-918-C100

In stock

198.00 USD

Variant

0.1 mg

11-918-C100

In stock

198.00 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Rabbit polyclonal

Specificity

The rabbit polyclonal antibody PAb (918) recognizes a red fluorescent protein tag mCherry.

Application

WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular staining or intracellular staining - depending on expression.
Western blotting: Recommended dilution: 1-2 μg/ml.

Immunogen

mCherry protein from Anaplasma marginale

Concentration

1 mg/ml

Preparation

Purified by ligand affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

The mCherry is a red fluorescent protein with excitation maximum 587 nm and emission maximum 610 nm. It has around 28 kDa, and it is being used as a fluorescent tag in expression systems.

UniProt ID X5DSL3

Western blotting analysis of mCherry fluorescent protein using rabbit polyclonal PAb (918) on lysates of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs (HEK293T/17 cells transfected with YFP/GPI; negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rabbit anti-mCherry polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for mCherry protein at approximately 30 kDa.

Flow cytometry surface staining pattern of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained using anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC).

Separation of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mCherry/YFP cell suspension.