Exbio — Research products — Antibodies — Tags and labels — Anti-GST tag Purified

Anti-GST tag Purified

Anti-GST tag Purified
Regulatory status
RUO
Antigen
GST tag
Clone
S-tag-05
Format
Purified
Reactivity
Tagged fusion proteins in all species
Application
IP, WB
Variant
0.1 mg
11-477-C100
In stock
150.00 USD
0.025 mg
11-477-C025
In stock
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD
0.1 mg
11-261-C100
In stock
75.00 USD
Contact distributor
Product details
Description
Images
Isotype
Mouse IgG2b
Specificity
The antibody S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibod is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems.
Application
IP, WB
Application details
Western blotting: Recommended dilution: 1 μg/ml; positive control: GST fusion protein, reducing conditions.
Reactivity
Tagged fusion proteins in all species
Immunogen
GST fusion protein
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Antigen description
The GST (glutathione-S-transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors.
11-477 WB
Western blotting analysis of isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli. Immunostained by anti-GST (S-tag-05). Lane 1: affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-Sepharose Lane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02; cat. no. 11-424-C100)
Variant
0.1 mg
11-477-C100
In stock
150.00 USD
0.025 mg
11-477-C025
In stock
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD
0.1 mg
11-261-C100
In stock
75.00 USD
Contact distributor
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SDS download
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