Exbio — Research products — Antibodies — Tags and labels — Anti-GST tag Purified

Anti-GST tag Purified

Regulatory status

RUO

Antigen

GST tag

Clone

S-tag-05

Format

Purified

Reactivity

Tagged fusion proteins in all species

Application

IP, WB

Variant

0.1 mg

11-477-C100

In stock

165.00 USD

0.025 mg

11-477-C025

Delivery 1 week

82.50 USD

Variant

0.1 mg

11-477-C100

In stock

165.00 USD

0.025 mg

11-477-C025

Delivery 1 week

82.50 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG2b

Specificity

The antibody S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibod is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems.

Application

IP, WB

Application details

Western blotting: Recommended dilution: 1 μg/ml; positive control: GST fusion protein, reducing conditions.

Reactivity

Tagged fusion proteins in all species

Immunogen

GST fusion protein

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

The GST (glutathione-S-transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors.

Western blotting analysis of isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli. Immunostained by anti-GST (S-tag-05). Lane 1: affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-Sepharose Lane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02; cat. no. 11-424-C100)