Anti-GFP Purified

Anti-GFP Purified
Regulatory status
RUO
Antigen
GFP
Clone
PAb (476)
Format
Purified
Reactivity
Tagged fusion proteins in all species
Application
Variant
0.1 mg
11-476-C100
In stock
150.00 USD

0.025 mg
11-476-C025
Delivery 1 week
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD

0.1 mg
11-261-C100
In stock
75.00 USD
Product details
Description
Images
References
Isotype
Rabbit polyclonal
Specificity
The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species.
Application
Application details
Western blotting: Recommended dilution 0.5-1.5 μg/ml; positive control: transfected cells; negative control: non-transfected cells.
Immunocytochemistry: Recommended dilution 1-3 μg/ml.
Reactivity
Tagged fusion proteins in all species
Immunogen
EGFP, a native full-length protein
Concentration
1 mg/ml
Preparation
Purified by ligand affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Green fluorescent protein, EGFP, EYFP
Antigen description
Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (λ=509 nm) when excited (excitation by Blue or UV light, absorption peak λ=395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chomophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. yellow fluorescent protein, cyan fluorescent protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.
UniProt ID P42212
11-476_ICC
Immunocytochemistry staining (confocal microscopy) of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP; top) or nuclear Polycomb 2-EYFP fusion protein (Pc2-EYFP; bottom). The natural fluorescence of the produced proteins is shown in the green channel (left), polyclonal anti-GFP antibody signal was detected in the red channel (right). The system was carefully tested for overlap of these two optical channels and images were scanned separately in sequential scanning mode. The blue nuclear stain is also shown.
11-476 IP
Immunoprecipitation of GFP-NLS from HEK293 cells using anti-GFP antibody. HEK293 cells were transfected with expression construct encoding GFP-NLS protein. Twenty hours post transfection cells were lysed in non-denaturating conditions (Lysis buffer: 20 mM Tris, pH 7.5, 100 mM NaCl, 0.5% Triton X-100, inhibitors of proteases). Aliquots of cell lysate were immunoprecipitated using a polyclonal anti-GFP antibody (lane 2) or a pre-immune rabbit serum (lane 3). Immunoprecipitates together with a sample of the cell lysate (lane 1) were separated on SDS-PAGE polyacrylamide gel and immunoblotted with the anti-GFP antibody. The positions of molecular weight markers in kDa are indicated at the left.

Product specific references:

Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun 7;25(11):2326-37.
PubMed
Variant
0.1 mg
11-476-C100
In stock
150.00 USD

0.025 mg
11-476-C025
Delivery 1 week
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD

0.1 mg
11-261-C100
In stock
75.00 USD