Anti-Dendra2 purified

Anti-Dendra2 purified
Regulatory status
RUO
Antigen
Dendra2
Clone
PAb (836)
Format
Purified
Reactivity
Species independent
Variant
0.1 mg
11-836-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Product details
Description
Images
References
Isotype
Rabbit polyclonal
Specificity
The rabbit polyclonal antibody PAb (836) recognizes Dendra2 tag, a green-to red photoswitchable protein derived from an octocoral (Dendronephthya).
Application details
Western blotting: Recommended dilution: 1-2 μg/ml.
Flow cytometry: Recommended dilution: 1-4 μg/ml. Extracellular and intracellular staining.
Reactivity
Species independent
Immunogen
Dendra2 tag
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Dendra2 tag
Antigen description
Dendra2 is an improved version of green-to-red photoswitchable protein Dendra, and compared to it, Dendra2 exhibits brighter fluorescence before and after photoswitching. Excitation maximum of Dendra2 is 490 nm before and 553 nm after photoactivation, and its emission maximum is 507 nm before and 573 nm after photoactivation. Activating light for Dendra2 is UV/violet to blue. Nonactivated Dendra2 spectral characteristics are similar to EGFP, and this green fluorescence can be detected at low light intensities of blue light. At hight intensities of the same blue light (or of UV/violet light) Dendra2 is photoactivated and gets emission characteristics similar to TRITC.
11-836_WB
Western blotting analysis of Dendra2/GPI fusion protein using rabbit polyclonal antibody PAb (836) on lysates of HEK293T/17 cells transfected with Dendra2/GPI construct; reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of anti-Dendra2 rabbit polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for Dendra2/GPI fusion protein at approximately 35 kDa.
11-836_FC_Dot-plot
Flow cytometry surface staining pattern of HEK293T/17 cells transfected with Dendra2/GPI construct stained using anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC).
11-836_FC_Histogram
Separation of HEK293T/17 cells transfected with Dendra2/GPI construct stained anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC, red-filled) from HEK293T/17 cells transfected with Dendra2/GPI construct unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/Dendra2/GPI cell suspension.

Product specific references:

Patrizio A, Renner M, Pizzarelli R, Triller A, Specht CG: Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers. Sci Rep. 2017 Sep 7;7(1):10899.
PubMed
McEwen AE, Maher MT, Mo R, Gottardi CJ: E-cadherin phosphorylation occurs during its biosynthesis to promote its cell surface stability and adhesion. Mol Biol Cell. 2014 Aug 15;25(16):2365-74.
PubMed
Variant
0.1 mg
11-836-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

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