Exbio — Research products — Antibodies — Tags and labels — Anti-DDDDK tag Purified

Anti-DDDDK tag Purified

Regulatory status

RUO

Antigen

DDDDK tag

Clone

F-tag-01

Format

Purified

Reactivity

Tagged fusion proteins in all species

Application

WB, ICC

Variant

0.1 mg

11-425-C100

In stock

165.00 USD

0.025 mg

11-425-C025

Delivery 1 week

82.50 USD

Variant

0.1 mg

11-425-C100

In stock

165.00 USD

0.025 mg

11-425-C025

Delivery 1 week

82.50 USD

Contact distributor

Product details

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody F-tag-01 recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.

Application

WB, ICC

Reactivity

Tagged fusion proteins in all species

Immunogen

Synthetic peptide: DYKDDDDK conjugated to KLH

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594) B - cell nuclei stained with DAPI (blue) C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)

Product specific references:

Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun 7;25(11):2326-37.
PubMed

Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V: Dazap2 modulates transcription driven by the Wnt effector TCF-4. Nucleic Acids Res. 2009 Mar 20. [Epub ahead of print]
PubMed