Anti-DDDDK tag Purified

Anti-DDDDK tag Purified
Regulatory status
RUO
Antigen
DDDDK tag
Clone
F-tag-01
Format
Purified
Reactivity
Tagged fusion proteins in all species
Application
WB, ICC
Variant
0.1 mg
11-425-C100
In stock

0.025 mg
11-425-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody F-tag-01 recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.
Application
WB, ICC
Reactivity
Tagged fusion proteins in all species
Immunogen
Synthetic peptide: DYKDDDDK conjugated to KLH
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-425 ICC
Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594) B - cell nuclei stained with DAPI (blue) C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)

Product specific references:

Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun 7;25(11):2326-37.
PubMed
Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V: Dazap2 modulates transcription driven by the Wnt effector TCF-4. Nucleic Acids Res. 2009 Mar 20. [Epub ahead of print]
PubMed
Variant
0.1 mg
11-425-C100
In stock

0.025 mg
11-425-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock