Exbio — Research products — Antibodies — Tags and labels — Anti-c-Myc tag FITC

Anti-c-Myc tag FITC

Anti-c-Myc tag FITC
Regulatory status
RUO
Antigen
c-Myc tag
Clone
9E10
Format
FITC
Reactivity
Tagged fusion proteins in all species, Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
0.1 mg
1F-433-C100
In stock
225.00 USD
0.025 mg
1F-433-C025
Out of stock
112.50 USD
Variant
0.1 mg
11-261-C100
In stock
225.00 USD
0.1 mg
11-261-C100
In stock
112.50 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody 9E10 can be used to detect the c-Myc tag.
Application
FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml; membrane permeabilization is required.
Reactivity
Tagged fusion proteins in all species, Human
Immunogen
Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
Other names
bHLH, MRTL, MYCC
Concentration
1 mg/ml
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1F-433 FC
Flow cytometry analysis of transfected LST-1-c-Myc in HEK-293 cells (red) compared with nontransfected HEK-293 cells (blue) using mouse monoclonal anti-c-Myc (9E10) FITC.
1F-433 FC_2
Flow cytometry analysis (intracellular staining) of transfected LST-1-c-Myc in HEK-293 cells (red-filled) compared with nontransfected HEK-293 cells (black-dashed) using mouse monoclonal anti-c-Myc (9E10) FITC.

General references:

Persson H, Hennighausen L, Taub R, DeGrado W, Leder P: Antibodies to human c-myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation. Science. 1984 Aug 17;225(4663):687-93.
PubMed
Evan GI et al. 1985. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol 5:3610-6.
Spandidos DA et al. 1987. Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue. Anticancer Res 7:1299-304.
Prendergast GC. 1999. Mechanisms of apoptosis by c-Myc. Oncogene 18(19):2967-2987.
Dang CV, Resar LM, Emison E, Kim S, Li Q, Prescott JE, Wonsey D, Zeller K. 1999. Function of the c-Myc oncogenic transcription factor. Exp Cell Res 253(1): 63-77.
Boxer LM, Dang CV. 2001. Translocations involving c-myc and c-myc function. Oncogene 20(40):5595-5610.
Hoffman B, Amanullah A, Shafarenko M, Liebermann DA. 2002. The proto-oncogene c-myc in hematopoietic development and leukemogenesis. Oncogene 21(21): 3414-3421.

Product specific references:

Wang X, Campoli M, Ko E, Luo W, Ferrone S: Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies. J Immunol Methods. 2004 Nov;294(1-2):23-35.
PubMed
Hilpert K, Hansen G, Wessner H, Kuttner G, Welfle K, Seifert M, Hohne W: Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose. Protein Eng. 2001 Oct;14(10):803-6.
PubMed
Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61.
PubMed
Jelínková I, Šafaříková B, Vondálová Blanářová O, Skender B, Hofmanová J, Sova P, Moyer MP, Kozubík A, Kolář Z, Ehrmann J, Hyršlová Vaculová A: Biochem Pharmacol. 2014 Dec 1;92(3):415-24.
PubMed
Siegel J, Brandner G, Hess RD: Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC. Int J Oncol. 1998 Dec;13(6):1259-62.
PubMed
Veracini L, Simon V, Richard V, Schraven B, Horejsi V, Roche S, Benistant C: The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1. J Cell Biol. 2008 Aug 11;182(3):603-14.
PubMed
Baggio R, Burgstaller P, Hale SP, Putney AR, Lane M, Lipovsek D, Wright MC, Roberts RW, Liu R, Szostak JW, Wagner RW: Identification of epitope-like consensus motifs using mRNA display. J Mol Recognit. 2002 May-Jun;15(3):126-34.
PubMed
Fujiwara K, Poikonen K, Aleman L, Valtavaara M, Saksela K, Mayer BJ: A single-chain antibody/epitope system for functional analysis of protein-protein interactions. Biochemistry. 2002 Oct 22;41(42):12729-38.
PubMed
Svadlenka J, Brazina J, Hanzlikova H, Cermak L, Andera L: Multifunctional adaptor protein Daxx interacts with chromatin-remodelling ATPase Brg1. Biochem Biophys Rep. 2015 Dec 30;5:246-252.
PubMed
Variant
0.1 mg
1F-433-C100
In stock
225.00 USD
0.025 mg
1F-433-C025
Out of stock
112.50 USD
Variant
0.1 mg
11-261-C100
In stock
225.00 USD
0.1 mg
11-261-C100
In stock
112.50 USD
Contact distributor
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