Anti-Phosphotyrosine Purified

Anti-Phosphotyrosine Purified
Regulatory status
RUO
Antigen
Phosphotyrosine
Clone
P-Tyr-01
Format
Purified
Reactivity
Species independent
Variant
0.1 mg
11-263-C100
In stock
176.00 USD

0.025 mg
11-263-C025
Delivery 1 week
88.00 USD
Variant
0.1 mg
11-263-C100
In stock
176.00 USD

0.025 mg
11-263-C025
Delivery 1 week
88.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.
Application details
Western blotting: Recommended dilution: 1-2 μg/ml. Flow cytometry: Recommended dilution: 2-5 μg/ml. Intracellular staining.
Reactivity
Species independent
Immunogen
Phosphotyrosine conjugated to bovine serum albumin.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
PY
11-263_FC_Histogram
Anti-Phosphotyrosine purified antibody (clone P-Tyr-01) Specificity Verification by Flow Cytometry

Anti-Phosphotyrosine purified antibody (concentration in sample 2 μg/ml, GAM FITC, red-filled histogram) binds specifically to surface phosphotyrosines in EGF treated A431 cells (upper panel), but not to the untreated A431 cells (lower panel).
Level of non-specific binding was assessed using Mouse IgG1 isotype control purified antibody (MOPC-21) under same conditions (concentration in sample 2 μg/ml, GAM FITC, black-dashed histogram).

Product specific references:

Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. Elife. 2015 Dec 9;4:e10766.
PubMed
Brdicka T, Imrich M, Angelisová P, Brdicková N, Horváth O, Spicka J, Hilgert I, Lusková P, Dráber P, Novák P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsí V: Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16;196(12):1617-26.
PubMed
Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol. 2007 Feb 15;178(4):2262-71.
PubMed
Brdicka T, Pavlistova D, Leo A, Bruyns E, Korinek V, Angelisova P, Scherer J, Shevchenko A, Hilgert I, Cerny J, Drbal K, Kuramitsu Y, Kornacker B, Horejsi V, Schraven B: Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000 May 1;191(9):1591-604.
PubMed
Variant
0.1 mg
11-263-C100
In stock
176.00 USD

0.025 mg
11-263-C025
Delivery 1 week
88.00 USD
Variant
0.1 mg
11-263-C100
In stock
176.00 USD

0.025 mg
11-263-C025
Delivery 1 week
88.00 USD

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