Exbio — Research products — Antibodies — Small organic molecules — Anti-Phosphotyrosine Alexa Fluor<sup>®</sup> 647

Anti-Phosphotyrosine Alexa Fluor^® 647

Regulatory status

RUO

Antigen

Phosphotyrosine

Clone

P-Tyr-01

Format

Alexa Fluor^® 647

Reactivity

Species independent

Application

WB, FC (QC tested), ICC

Excitation laser

red (633 nm)

Variant

0.1 mg

A6-263-C100

In stock

242.00 USD

0.025 mg

A6-263-C025

Delivery 1 week

121.00 USD

Variant

0.1 mg

A6-263-C100

In stock

242.00 USD

0.025 mg

A6-263-C025

Delivery 1 week

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.

Application

WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.

Reactivity

Species independent

Immunogen

Phosphotyrosine conjugated to bovine serum albumin.

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

Separation of phosphotyrosine positive cells (red-filled) from phosphotyrosine negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of EGF stimulated A431 cells stained using anti-phosphotyrosine (P-Tyr-01) Alexa Fluor® 647 antibody (concentration in sample 3 μg/ml).

Product specific references:

Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. Elife. 2015 Dec 9;4:e10766.
PubMed

Brdicka T, Imrich M, Angelisová P, Brdicková N, Horváth O, Spicka J, Hilgert I, Lusková P, Dráber P, Novák P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsí V: Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16;196(12):1617-26.
PubMed

Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol. 2007 Feb 15;178(4):2262-71.
PubMed

Brdicka T, Pavlistova D, Leo A, Bruyns E, Korinek V, Angelisova P, Scherer J, Shevchenko A, Hilgert I, Cerny J, Drbal K, Kuramitsu Y, Kornacker B, Horejsi V, Schraven B: Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000 May 1;191(9):1591-604.
PubMed