Exbio — Research products — Antibodies — Small organic molecules — Anti-Nitrotyrosine Purified

Anti-Nitrotyrosine Purified

Regulatory status

RUO

Antigen

Nitrotyrosine

Clone

EM-30

Format

Purified

Reactivity

Species independent

Application

WB, IHC-F, IHC-P

Variant

0.1 mg

11-693-C100

In stock

293.70 USD

0.025 mg

11-693-C025

In stock

146.85 USD

Variant

0.1 mg

11-693-C100

In stock

293.70 USD

0.025 mg

11-693-C025

In stock

146.85 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG1

Specificity

The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues.

Application

WB, IHC-F, IHC-P

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia.
Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia.
Western blotting: positive control: NO2-BSA, NO2-HSA.

Reactivity

Species independent

Immunogen

NO2-Tyr-CH2-Thyroglobulin

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TyrNO2, C9H10N2O5

Antigen description

Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.