Anti-BrdU Purified

Anti-BrdU Purified
Regulatory status
RUO
Antigen
BrdU
Clone
MoBu-1
Format
Purified
Variant
0.1 mg
11-286-C100
In stock
165.00 USD

0.025 mg
11-286-C025
Delivery 1 week
82.50 USD
Variant
0.1 mg
11-286-C100
In stock
165.00 USD

0.025 mg
11-286-C025
Delivery 1 week
82.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It reacts also specifically with 5-bromouridine (BrU), and it does not cross-react with EdU.
Application details
Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining.
Immunocytochemistry: Recommended dilution: 2 μg/ml.
Immunohistochemistry (paraffin sections): Excellent.
Immunogen
5-bromodeoxyuridine conjugated with hemocyanine.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
5-bromodeoxyuridine
Antigen description
Bromodexyuridine (BrdU) is a thymidine analog, which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.
11-286 FC
Flow cytometry analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM human acute lymphoblastic leukemia cell line using purified anti-5-bromodeoxyuridin (MoBu-1) (detection by Goat anti-mouse IgG1 FITC). The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.
11-286_IHC
Immunohistochemistry staining of bromodeoxyuridine-labeled cells (chick embryo; paraffin-embedded sections) with anti-5-bromodeoxyuridine (MoBu-1).

General references:

Ashby J, Tinwell H, Soames A, Foster J: Induction of hyperplasia and increased DNA content in the uterus of immature rats exposed to coumestrol. Environ Health Perspect. 1999 Oct;107(10):819-22.
PubMed
Soames AR, Lavender D, Foster JR, Williams SM, Wheeldon EB: Image analysis of bromodeoxyuridine (BrdU) staining for measurement of S-phase in rat and mouse liver. J Histochem Cytochem. 1994 Jul;42(7):939-44.
PubMed

Product specific references:

Stanek D, Kiss T, Raska I: Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription. Eur J Cell Biol. 2000 Mar;79(3):202-7.
PubMed
Buckiova D, Kubinova L, Soukup A, Jelinek R, Brown NA: Hyperthermia in the chick embryo: HSP and possible mechanisms of developmental defects. Int J Dev Biol. 1998 Jul;42(5):737-40.
PubMed
Variant
0.1 mg
11-286-C100
In stock
165.00 USD

0.025 mg
11-286-C025
Delivery 1 week
82.50 USD
Variant
0.1 mg
11-286-C100
In stock
165.00 USD

0.025 mg
11-286-C025
Delivery 1 week
82.50 USD