Exbio — Research products — Antibodies — Small organic molecules — Anti-Biotin Alexa Fluor<sup>®</sup> 647

Anti-Biotin Alexa Fluor^® 647

Regulatory status

RUO

Antigen

Biotin

Clone

1D4-C5

Format

Alexa Fluor^® 647

Application

FC-IC, FC (QC tested)

Excitation laser

red (633 nm)

Variant

0.1 mg

A6-944-C100

In stock

248.60 USD

Variant

0.1 mg

A6-944-C100

In stock

248.60 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

Mouse monoclonal antibody 1D4-C5 recognizes biotin and can be used as a second-step reagent for detecting biotinylated antigens.

Application

FC-IC, FC (QC tested)

Application details

Flow cytometry: Recomended use: 1 μg/ml. Extracellular and intracellular staining.

Immunogen

biotinylated limulus hemocyanin

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

vitamin H, vitamin B7

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD4 (MEM-241) biotin antibody (concentration in sample 2 μg/ml) and secondary stained using anti-biotin (1D4-C5) Alexa Fluor® 647 (concentration in sample 0.19 µg/ml).

Product specific references:

Green NM, Moody KS, Debatis M, Marshak-Rothstein A: Activation of autoreactive B cells by endogenous TLR7 and TLR3 RNA ligands. J Biol Chem. 2012 Nov 16;287(47):39789-99.
PubMed

Uccellini MB, Busconi L, Green NM, Busto P, Christensen SR, Shlomchik MJ, Marshak-Rothstein A, Viglianti GA: Autoreactive B cells discriminate CpG-rich and CpG-poor DNA and this response is modulated by IFN-alpha. J Immunol. 2008 Nov 1;181(9):5875-84.
PubMed

Stensland ZC, Smith MJ: Enrichment and detection of antigen-binding B cells for mass cytometry. Magnetochemistry. 2021 Jul;7(7):92.
PubMed