Anti-Transferrin Purified Azide Free

Anti-Transferrin Purified Azide Free
Regulatory status
RUO
Antigen
Transferrin
Clone
HTF-14
Format
Purified Azide Free
Reactivity
Human, Pig, Rabbit
Application
ELISA, RIA, WB, IHC(P), ICC, FUNC
Variant
0.1 mg
10-258-C100
In stock

0.025 mg
10-258-C025
In stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
Application
ELISA, RIA, WB, IHC(P), ICC, FUNC
Reactivity
Human, Pig, Rabbit
Negative species
Horse, Dog, Sheep, Cow
Immunogen
Purified porcine transferrin.
Other names
TFQTL1, PRO1557, PRO2086
Preparation
Purified by precipitation and chromatography
Formulation
Azide free phosphate buffered saline (PBS), approx. pH 7.4
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
10-258 WB
Human transferrin detected by the mouse monoclonal antibody HTF-14. 1. hTransferrin; 5 µg/well (red. con.) 2. hTransferrin; 3 µg/well (red. con.) 3. hTransferrin; 1 µg/well (red. con.) M Low Range marker (Bio-Rad) 4. hTransferrin; 1 µg/well (non-red. con.) 5. hTransferrin; 3 µg/well (non-red. con.) 6. hTransferrin; 5 µg/well (non-red. con.)

General references:

Taketani S: Aquisition, mobilization and utilization of cellular iron and heme: endless findings and growing evidence of tight regulation. Tohoku J Exp Med. 2005 Apr;205(4):297-318.
PubMed
Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D: Liver iron transport. World J Gastroenterol. 2007 Sep 21;13(35):4725-36.
PubMed
Rouault TA: How mammals acquire and distribute iron needed for oxygen-based metabolism. PLoS Biol. 2003 Dec;1(3):E79
PubMed
Ghosh RN, Maxfield FR: Evidence for nonvectorial, retrograde transferrin trafficking in the early endosomes of HEp2 cells. J Cell Biol. 1995 Feb;128(4):549-61.
PubMed
Ghosh RN, Gelman DL, Maxfield FR: Quantification of low density lipoprotein and transferrin endocytic sorting HEp2 cells using confocal microscopy. J Cell Sci. 1994 Aug;107 ( Pt 8):2177-89.
PubMed
Hansen SH, Sandvig K, van Deurs B: Clathrin and HA2 adaptors: effects of potassium depletion, hypertonic medium, and cytosol acidification. J Cell Biol. 1993 Apr;121(1):61-72.
PubMed

Product specific references:

Bartek J, Viklicky V, Franek F, Angelisova P, Draber P, Jarosikova T, Nemec M, Verlova H: Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity. Immunol Lett. 1982 May;4(5):231-5.
PubMed
Nováková M, Dráberová E, Schürmann W, Czihak G, Viklický V, Dráber P: gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies. Cell Motil Cytoskeleton. 1996;33(1):38-51.
PubMed
Bartek J, Viklicky V, Stratil A: Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding. Br J Haematol. 1985 Mar;59(3):435-41.
PubMed
Variant
0.1 mg
10-258-C100
In stock

0.025 mg
10-258-C025
In stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock