Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Transferrin Purified

Anti-Transferrin Purified

Regulatory status

RUO

Antigen

Transferrin

Clone

HTF-14

Format

Purified

Reactivity

Human, Pig, Rabbit

Application

ELISA, RIA, WB, IHC(P), ICC

Variant

0.1 mg

11-258-C100

In stock

130.00 USD

0.025 mg

11-258-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-258-C100

In stock

130.00 USD

0.025 mg

11-258-C025

Delivery 1 week

65.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.

Application

ELISA, RIA, WB, IHC(P), ICC

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: placenta.
Western blotting: non-reducing conditions, recommended dilution: 1-2 μg/ml.

Reactivity

Human, Pig, Rabbit

Negative species

Horse, Dog, Sheep, Cow

Immunogen

Purified porcine transferrin.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TFQTL1, PRO1557, PRO2086

Antigen description

Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.

Entrez Gene ID 7018

UniProt ID P02787

Western blotting detection of human transferrin by mouse monoclonal antibody HTF-14. 1. hTransferrin; 5 µg/well (red. con.) 2. hTransferrin; 3 µg/well (red. con.) 3. hTransferrin; 1 µg/well (red. con.) M Low Range marker (Bio-Rad) 4. hTransferrin; 1 µg/well (non-red. con.) 5. hTransferrin; 3 µg/well (non-red. con.) 6. hTransferrin; 5 µg/well (non-red. con.)

Immunohistochemistry staining of human placenta (paraffin sections) using anti-transferrin (HTF-14). Commercially tested by LifeSpan BioSciences.

General references:

Taketani S: Aquisition, mobilization and utilization of cellular iron and heme: endless findings and growing evidence of tight regulation. Tohoku J Exp Med. 2005 Apr;205(4):297-318.
PubMed

Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D: Liver iron transport. World J Gastroenterol. 2007 Sep 21;13(35):4725-36.
PubMed

Rouault TA: How mammals acquire and distribute iron needed for oxygen-based metabolism. PLoS Biol. 2003 Dec;1(3):E79
PubMed

Ghosh RN, Maxfield FR: Evidence for nonvectorial, retrograde transferrin trafficking in the early endosomes of HEp2 cells. J Cell Biol. 1995 Feb;128(4):549-61.
PubMed

Ghosh RN, Gelman DL, Maxfield FR: Quantification of low density lipoprotein and transferrin endocytic sorting HEp2 cells using confocal microscopy. J Cell Sci. 1994 Aug;107 ( Pt 8):2177-89.
PubMed

Hansen SH, Sandvig K, van Deurs B: Clathrin and HA2 adaptors: effects of potassium depletion, hypertonic medium, and cytosol acidification. J Cell Biol. 1993 Apr;121(1):61-72.
PubMed

Product specific references:

Bartek J, Viklicky V, Franek F, Angelisova P, Draber P, Jarosikova T, Nemec M, Verlova H: Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity. Immunol Lett. 1982 May;4(5):231-5.
PubMed

Nováková M, Dráberová E, Schürmann W, Czihak G, Viklický V, Dráber P: gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies. Cell Motil Cytoskeleton. 1996;33(1):38-51.
PubMed

Bartek J, Viklicky V, Stratil A: Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding. Br J Haematol. 1985 Mar;59(3):435-41.
PubMed

Viklický V, Bártek J: Utilization of monoclonal antibodies for mapping of antigenic determinants on the transferrin molecule. EOS n. 4, vol. 4, 1984, 263-267.