Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-TNF alpha Purified

Anti-TNF alpha Purified

Regulatory status

RUO

Antigen

TNF alpha

Clone

MAb1

Format

Purified

Reactivity

Human

Application

ELISA, WB

Variant

0.1 mg

11-687-C100

In stock

143.00 USD

0.025 mg

11-687-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-687-C100

In stock

143.00 USD

0.025 mg

11-687-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody MAb1 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).

Application

ELISA, WB

Application details

ELISA: It can be used as capture antibody in combination with biotinylated antibody MAb11.

Reactivity

Human

Immunogen

Recombinant human TNF-alpha

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TNFA, DIF, TNLG1F

Antigen description

TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.

Entrez Gene ID 7124

UniProt ID P01375

ELISA analysis of TNF alpha (0 - 4.1 ng/ml) using capture antibody MAb1 (5 μg/ml) and biotinylated detection antibody MAb11 (0.15 - 10 μg/ml).

Product specific references:

Cesaro-Tadic S, Dernick G, Juncker D, Buurman G, Kropshofer H, Michel B, Fattinger C, Delamarche E: High-sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems. Lab Chip. 2004 Dec;4(6):563-9.
PubMed

Visser J, Graffelman W, Blauw B, Haspels I, Lentjes E, de Kloet ER, Nagelkerken L: LPS-induced IL-10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone. J Neuroimmunol. 2001 Oct 1;119(2):343-9.
PubMed

Yan SR, Qing G, Byers DM, Stadnyk AW, Al-Hertani W, Bortolussi R: Role of MyD88 in diminished tumor necrosis factor alpha production by newborn mononuclear cells in response to lipopolysaccharide. Infect Immun. 2004 Mar;72(3):1223-9.
PubMed

Wahlström J, Katchar K, Wigzell H, Olerup O, Eklund A, Grunewald J: Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in sarcoidosis. Am J Respir Crit Care Med. 2001 Jan;163(1):115-21.
PubMed

Lindholm C, Naylor A, Johansson EL, Quiding-Järbrink M: Mucosal vaccination increases endothelial expression of mucosal addressin cell adhesion molecule 1 in the human gastrointestinal tract. Infect Immun. 2004 Feb;72(2):1004-9.
PubMed

Ahlstedt I, Lindholm C, Lönroth H, Hamlet A, Svennerholm AM, Quiding-Järbrink M: Role of local cytokines in increased gastric expression of the secretory component in Helicobacter pylori infection. Infect Immun. 1999 Sep;67(9):4921-5.
PubMed