Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-TNF alpha FITC

Anti-TNF alpha FITC

Anti-TNF alpha FITC
Regulatory status
RUO
Antigen
TNF alpha
Clone
MAb11
Format
FITC
Reactivity
Pig, Human, Non-human primates
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1F-688-T100
In stock
0.00 USD
25 tests
1F-688-T025
Out of stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD
0.1 mg
11-261-C100
In stock
0.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Pig, Human, Non-human primates
Immunogen
Recombinant human TNF-alpha
Other names
TNFA, DIF, TNLG1F
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1F-688_FC_Profil Značení
Flow cytometry intracellular staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human TNF alpha (MAb11) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).
1F-688_FC_Dot-plot
Flow cytometry multicolor intracellular staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human TNF alpha (MAb11) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension). For surface staining was used anti-human CD3 (UCHT1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
1F-688_FC_Histogram
Separation of human TNF alpha positive CD3 positive cells (red-filled) from TNF alpha negative CD3 negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human TNF alpha (MAb11) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

Product specific references:

Cesaro-Tadic S, Dernick G, Juncker D, Buurman G, Kropshofer H, Michel B, Fattinger C, Delamarche E: High-sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems. Lab Chip. 2004 Dec;4(6):563-9.
PubMed
Attarbaschi T, Willheim M, Ramharter M, Hofmann A, Wahl K, Winkler H, Graninger W, Winkler S: T cell cytokine profile during primary Epstein-Barr virus infection (infectious mononucleosis). Eur Cytokine Netw. 2003 Jan-Mar;14(1):34-9.
PubMed
Wahlström J, Katchar K, Wigzell H, Olerup O, Eklund A, Grunewald J: Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in sarcoidosis. Am J Respir Crit Care Med. 2001 Jan;163(1):115-21.
PubMed
Yan SR, Qing G, Byers DM, Stadnyk AW, Al-Hertani W, Bortolussi R: Role of MyD88 in diminished tumor necrosis factor alpha production by newborn mononuclear cells in response to lipopolysaccharide. Infect Immun. 2004 Mar;72(3):1223-9.
PubMed
Visser J, Graffelman W, Blauw B, Haspels I, Lentjes E, de Kloet ER, Nagelkerken L: LPS-induced IL-10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone. J Neuroimmunol. 2001 Oct 1;119(2):343-9.
PubMed
Variant
100 tests
1F-688-T100
In stock
0.00 USD
25 tests
1F-688-T025
Out of stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD
0.1 mg
11-261-C100
In stock
0.00 USD
Contact distributor
Datasheet download
SDS download
Bulk requests
Technical support for this product

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