Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-TNF alpha FITC

Anti-TNF alpha FITC

Regulatory status

RUO

Antigen

TNF alpha

Clone

MAb11

Format

FITC

Reactivity

Pig, Human, Non-human primates

Application

FC-IC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1F-688-T100

In stock

203.50 USD

25 tests

1F-688-T025

Delivery 1 week

101.75 USD

Variant

100 tests

1F-688-T100

In stock

203.50 USD

25 tests

1F-688-T025

Delivery 1 week

101.75 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).

Application

FC-IC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.

Reactivity

Pig, Human, Non-human primates

Immunogen

Recombinant human TNF-alpha

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TNFA, DIF, TNLG1F

Antigen description

TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.

Entrez Gene ID 7124

UniProt ID P01375

Flow cytometry intracellular staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human TNF alpha (MAb11) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

Flow cytometry multicolor intracellular staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human TNF alpha (MAb11) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension). For surface staining was used anti-human CD3 (UCHT1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Separation of human TNF alpha positive CD3 positive cells (red-filled) from TNF alpha negative CD3 negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human TNF alpha (MAb11) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

Product specific references:

Cesaro-Tadic S, Dernick G, Juncker D, Buurman G, Kropshofer H, Michel B, Fattinger C, Delamarche E: High-sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems. Lab Chip. 2004 Dec;4(6):563-9.
PubMed

Attarbaschi T, Willheim M, Ramharter M, Hofmann A, Wahl K, Winkler H, Graninger W, Winkler S: T cell cytokine profile during primary Epstein-Barr virus infection (infectious mononucleosis). Eur Cytokine Netw. 2003 Jan-Mar;14(1):34-9.
PubMed

Wahlström J, Katchar K, Wigzell H, Olerup O, Eklund A, Grunewald J: Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in sarcoidosis. Am J Respir Crit Care Med. 2001 Jan;163(1):115-21.
PubMed

Yan SR, Qing G, Byers DM, Stadnyk AW, Al-Hertani W, Bortolussi R: Role of MyD88 in diminished tumor necrosis factor alpha production by newborn mononuclear cells in response to lipopolysaccharide. Infect Immun. 2004 Mar;72(3):1223-9.
PubMed

Visser J, Graffelman W, Blauw B, Haspels I, Lentjes E, de Kloet ER, Nagelkerken L: LPS-induced IL-10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone. J Neuroimmunol. 2001 Oct 1;119(2):343-9.
PubMed