Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-PSA Purified

Anti-PSA Purified

Regulatory status

RUO

Antigen

PSA

Clone

A67-B/E3

Format

Purified

Reactivity

Human

Application

ELISA, WB, IHC(P)

Variant

0.1 mg

11-289-C100

In stock

121.00 USD

0.025 mg

11-289-C025

Delivery 1 week

60.50 USD

Variant

0.1 mg

11-289-C100

In stock

121.00 USD

0.025 mg

11-289-C025

Delivery 1 week

60.50 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody A67-B/E3 reacts with prostate-specific antigen (PSA), a protein produced by normal prostate cells. The highest amounts of PSA are found in the seminal fluid. This antibody recognizes both free and complexed PSA.

Workshop

TD-3 PSA Workshop

Application

ELISA, WB, IHC(P)

Application details

Western blotting: Positive material: seminal plasma, reducing conditions, recommended antibody dilution: 2-5 μg/ml.

Reactivity

Human

Immunogen

30 kDa protein fraction prepared from human seminal plasma

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Prostate-specific antigen, Kallikrein-3, Semenogelase, Seminin

Antigen description

Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. This enzyme participates in the dissolution of the seminal fluid coagulum and plays an important role in fertility. The highest amounts of PSA are found in the seminal fluid; some PSA escapes the prostate and can be found in the serum. This serum component has been used to track the response to therapy in men with prostate cancer.

Entrez Gene ID 354

UniProt ID P07288

Immunohistochemistry staining of prostate (paraffin-embedded sections) with anti-human Prostate-specific antigen (A67-B/E3).

Immunohistochemistry staining of human prostate (paraffin-embedded sections) with anti-PSA (A67-B/E3), 10 μg/ml. Commercially tested by LifeSpan BioSciences.

Western blotting analysis of human PSA using mouse monoclonal antibody A67-B/E3 on human seminal plasma under reducing conditions. Nitrocellulose membrane was probed with 5 µg/ml of mouse anti-PSA monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. PSA was detected at approximately 30 kDa.