Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Por Transferrin Purified

Anti-Por Transferrin Purified

Regulatory status

RUO

Antigen

Transferrin

Clone

PTF-02

Format

Purified

Reactivity

Pig

Application

ELISA, WB

Variant

0.1 mg

11-475-C100

In stock

143.00 USD

0.025 mg

11-475-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-475-C100

In stock

143.00 USD

0.025 mg

11-475-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody PTF-02 recognizes porcine serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.

Application

ELISA, WB

Reactivity

Pig

Negative species

Horse, Sheep

Immunogen

Porcine transferrin

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TFQTL1, PRO1557, PRO2086

Antigen description

Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.

Entrez Gene ID 396996

UniProt ID P09571

Western blotting analysis (reducing conditions) of Transferrin of various species using anti-porcine Transferrin (PTF-02). Lane 1: equine transferrin Lane 2: sheep transferrin Lane 3: porcine transferrin

General references:

D. R. Hall, J. M. Hadden, G. A. Leonard, S. Bailey, M. Neu, M. Winn and P. F. Lindley: The crystal and molecular structures of diferric porcine and rabbit serum transferrins at resolutions of 2.15 and 2.60 Å, respectively. Acta Cryst. (2002). D58, 70-80
PubMed

Product specific references:

Trebichavsky I, Macku B, Viklicky V, Koukal M, Siman P: Monoclonal antibodies against pig transferrin. Blocking and binding activity. Folia Microbiol (Praha). 1987;32(5):448-52.
PubMed