Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Perforin PE

Anti-Perforin PE

Anti-Perforin PE
Regulatory status
RUO
Antigen
Perforin
Clone
dG9
Format
PE
Reactivity
Human, Cow
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1P-765-T100
In stock
220.00 USD
25 tests
1P-765-T025
In stock
110.00 USD
Variant
100 tests
1P-765-T100
In stock
220.00 USD
25 tests
1P-765-T025
In stock
110.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2b kappa
Specificity
The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
Reactivity
Human, Cow
Negative species
Mouse
Immunogen
purified granules from human YT lymphoma cell line
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
PRF1, P1, PFP, HPLH2
Antigen description
Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.
Entrez Gene ID 5551
UniProt ID P14222
1P-765 FC
Flow cytometry analysis (intracellular staining) of perforin in human peripheral blood cells with anti-perforin (dG9) PE.

Product specific references:

Vargas-Inchaustegui DA, Demberg T, Robert-Guroff M: A CD8α(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities. Immunology. 2011 Nov;134(3):326-40.
PubMed
Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. J Mol Cell Biol. 2010 Jun;2(3):139-51.
PubMed
Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW: Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions. PLoS Pathog. 2012;8(11):e1002984.
PubMed
Hersperger AR, Makedonas G, Betts MR: Flow cytometric detection of perforin upregulation in human CD8 T cells. Cytometry A. 2008 Nov;73(11):1050-7.
PubMed
Hameed A, Olsen KJ, Cheng L, Fox WM, Hruban RH, Podack ER: Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am J Pathol. 1992 May;140(5):1025-30.
PubMed
Variant
100 tests
1P-765-T100
In stock
220.00 USD
25 tests
1P-765-T025
In stock
110.00 USD
Variant
100 tests
1P-765-T100
In stock
220.00 USD
25 tests
1P-765-T025
In stock
110.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product