Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Lysozyme Purified

Anti-Lysozyme Purified

Regulatory status

RUO

Antigen

Lysozyme

Clone

LZ598-10G9

Format

Purified

Reactivity

Human

Application

MC (CyTOF), FC (QC tested), ICC

Variant

0.1 mg

11-800-C100

In stock

143.00 USD

0.025 mg

11-800-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-800-C100

In stock

143.00 USD

0.025 mg

11-800-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody LZ598-10G9 recognizes lysozyme, an approximately 17 kDa antibacterial enzyme, which is being used as a marker for the lineage diagnosis of acute leukemias (intracellular antigen).

Application

MC (CyTOF), FC (QC tested), ICC

Application details

Flow cytometry: Intracellular staining; recommended dilution: 1-12 µg/ml. Excellent.
Immunohistochemistry (paraffin sections): Positive tissue: tonsil.

Reactivity

Human

Immunogen

human lysozyme

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

LZM, LYZF1

Antigen description

Lysozyme is anti-bacterial enzyme found mainly in milk, saliva, tears, plasma, spleen, mucus, and leukocytes (e.g. in cytoplasmic granules of neutrophils). It damages bacterial cell walls by hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. Lysozyme is part of the innate immune system. It protects wet body surfaces, such as conjunctiva. Reduced lysozyme levels have been associated with bronchopulmonary dysplasia in newborns. On the other hand high lysozyme blood levels produced for example by myelomonocytic leukemia cells can lead to kidney failure and low blood potassium.

Entrez Gene ID 4069

UniProt ID P61626

Flow cytometry analysis (intracellular staining) of lysozyme in human peripheral blood with anti-lysozyme (LZ598-10G9) purified, GAM-FITC.

Immunocytochemistry staining of lysozyme (green; A, C) in Hep G2 cells (A, B, C) using mouse monoclonal antibody LZ598-10G9. Actin decorated with phalloidin (red), DNA stained with DAPI (blue). Figure D represents SK-BR-3 cells with no expression of lysozyme, stained with LZ598-10G9 antibody (no background signal).

Mass cytometry (intracellular staining) of PBMC after Ficoll-Paque separation with anti-human lysozyme (LZ598-10G9) Gd155. Gated on native.

General references:

Strobl H, Knapp W: Myeloid cell-associated lysosomal proteins as flow cytometry markers for leukocyte lineage classification. J Biol Regul Homeost Agents. 2004 Jul-Dec;18(3-4):335-9.
PubMed