Anti-IFN gamma PerCP-Cy™5.5

Anti-IFN gamma PerCP-Cy™5.5
Regulatory status
RUO
Antigen
IFN gamma
Clone
4S.B3
Format
PerCP-Cy™5.5
Reactivity
Non-human primates, Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
T9-706-T100
In stock
330.00 USD

25 tests
T9-706-T025
Delivery 1 week
165.00 USD
Variant
100 tests
T9-706-T100
In stock
330.00 USD

25 tests
T9-706-T025
Delivery 1 week
165.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 4S.B3 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.
Reactivity
Non-human primates, Human
Immunogen
Interferon gamma derived from human leukocytes
Preparation
Purified antibody is conjugated with activated tandem dye of activated Peridinin-Chlorophyll Protein-Cyanine 5.5 (PerCP-Cy™5.5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
Interferon gamma, IFN-gamma
Antigen description
The interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.
Entrez Gene ID 3458
UniProt ID P01579
T9-706_FC_Profil Značení
Flow cytometry intracellular staining pattern of human PHA stimulated and monesin treated peripheral blood mononuclear cells stained using anti-IFN gamma (4S.B3) PerCP-Cy™5.5 antibody (4 μl reagent per milion cells in 100 μl of cell suspension).
T9-706_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of PHA stimulated and monesin treated lymphocytes stained using anti-human CD3 (UCHT1) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood) and surface staining using anti-IFN gamma (4S.B3) PerCP-Cy™5.5 antibody (4 μl reagent per milion cells in 100 μl of cell suspension).
T9-706_FC_Histogram
Separation of human CD3 positive interferon gamma positive T cells (red-filled) from CD3 negative interferon gamma positive T cells (black-dashed) in flow cytometry analysis (intracellular staining) of PHA stimulated and monesin treated peripheral blood mononuclear cells stained using anti-IFN gamma (4S.B3) PerCP-Cy™5.5 antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

General references:

Schroder K, Hertzog PJ, Ravasi T, Hume DA: Interferon-gamma: an overview of signals, mechanisms and functions. J Leukoc Biol. 2004 Feb;75(2):163-89.
PubMed
Ellis TN, Beaman BL: Interferon-gamma activation of polymorphonuclear neutrophil function. Immunology. 2004 May;112(1):2-12.
PubMed
Costa-Pereira AP, Williams TM, Strobl B, Watling D, Briscoe J, Kerr IM: The antiviral response to gamma interferon. J Virol. 2002 Sep;76(18):9060-8.
PubMed
Okamura H, Kashiwamura S, Tsutsui H, Yoshimoto T, Nakanishi K: Regulation of interferon-gamma production by IL-12 and IL-18. Curr Opin Immunol. 1998 Jun;10(3):259-64.
PubMed
Young HA, Hardy KJ: Role of interferon-gamma in immune cell regulation. J Leukoc Biol. 1995 Oct;58(4):373-81. Review.
PubMed

Product specific references:

Coles AJ, Wing M, Smith S, Coraddu F, Greer S, Taylor C, Weetman A, Hale G, Chatterjee VK, Waldmann H, Compston A: Pulsed monoclonal antibody treatment and autoimmune thyroid disease in multiple sclerosis. Lancet. 1999 Nov 13;354(9191):1691-5.
PubMed
Meager A, Parti S, Barwick S, Spragg J, O'Hagan K: Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon. J Interferon Res. 1984 Fall;4(4):619-25.
PubMed
Caulfield JJ, Fernandez MH, Sousa AR, Lane SJ, Lee TH, Hawrylowicz CM: Regulation of major histocompatibility complex class II antigens on human alveolar macrophages by granulocyte-macrophage colony-stimulating factor in the presence of glucocorticoids. Immunology. 1999 Sep;98(1):104-10.
PubMed
Brattig NW, Lepping B, Timmann C, Büttner DW, Marfo Y, Hamelmann C, Horstmann RD: Onchocerca volvulus-exposed persons fail to produce interferon-gamma in response to O. volvulus antigen but mount proliferative responses with interleukin-5 and IL-13 production that decrease with increasing microfilarial density. J Infect Dis. 2002 Apr 15;185(8):1148-54.
PubMed
Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA: Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006 Jul;118(3):353-60.
PubMed
David A. Kosub, Ginger Lehrman, Jeffrey M. Milush, Dejiang Zhou, Elizabeth Chacko, Amanda Leone, Shari Gordon, Guido Silvestri, James G. Else, Philip Keiser, Mamta K. Jain, Donald L. Sodora: Gamma/Delta T-Cell Functional Responses Differ after Pathogenic Human Immunodeficiency Virus and Nonpathogenic Simian Immunodeficiency Virus Infections. J Virol. 2008 February; 82(3): 1155–1165.
PubMed
Erik L. Brincks, Tamara A. Kucaba, Kevin L. Legge, Thomas S. Griffith: Influenza-induced expression of functional TNF-related apoptosis-inducing ligand (TRAIL) on human PBMC. Hum Immunol. 2008 October; 69(10): 634–646.
PubMed
Variant
100 tests
T9-706-T100
In stock
330.00 USD

25 tests
T9-706-T025
Delivery 1 week
165.00 USD
Variant
100 tests
T9-706-T100
In stock
330.00 USD

25 tests
T9-706-T025
Delivery 1 week
165.00 USD

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