Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-IFN gamma FITC

Anti-IFN gamma FITC

Anti-IFN gamma FITC
Regulatory status
RUO
Antigen
IFN gamma
Clone
4S.B3
Format
FITC
Reactivity
Non-human primates, Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1F-706-T100
In stock
25 tests
1F-706-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock
0.1 mg
11-261-C100
In stock
Contact us
Product details
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 4S.B3 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Non-human primates, Human
Immunogen
Interferon gamma derived from human leukocytes
Other names
Interferon gamma, IFN-gamma
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
1F-706 FC
Flow cytometry analysis (intracellular staining) of IFN gamma in PHA-activated human PBMC with anti-IFN gamma (4S.B3) FITC.

General references:

Schroder K, Hertzog PJ, Ravasi T, Hume DA: Interferon-gamma: an overview of signals, mechanisms and functions. J Leukoc Biol. 2004 Feb;75(2):163-89.
PubMed
Ellis TN, Beaman BL: Interferon-gamma activation of polymorphonuclear neutrophil function. Immunology. 2004 May;112(1):2-12.
PubMed
Costa-Pereira AP, Williams TM, Strobl B, Watling D, Briscoe J, Kerr IM: The antiviral response to gamma interferon. J Virol. 2002 Sep;76(18):9060-8.
PubMed
Okamura H, Kashiwamura S, Tsutsui H, Yoshimoto T, Nakanishi K: Regulation of interferon-gamma production by IL-12 and IL-18. Curr Opin Immunol. 1998 Jun;10(3):259-64.
PubMed
Young HA, Hardy KJ: Role of interferon-gamma in immune cell regulation. J Leukoc Biol. 1995 Oct;58(4):373-81. Review.
PubMed

Product specific references:

Coles AJ, Wing M, Smith S, Coraddu F, Greer S, Taylor C, Weetman A, Hale G, Chatterjee VK, Waldmann H, Compston A: Pulsed monoclonal antibody treatment and autoimmune thyroid disease in multiple sclerosis. Lancet. 1999 Nov 13;354(9191):1691-5.
PubMed
Meager A, Parti S, Barwick S, Spragg J, O'Hagan K: Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon. J Interferon Res. 1984 Fall;4(4):619-25.
PubMed
Caulfield JJ, Fernandez MH, Sousa AR, Lane SJ, Lee TH, Hawrylowicz CM: Regulation of major histocompatibility complex class II antigens on human alveolar macrophages by granulocyte-macrophage colony-stimulating factor in the presence of glucocorticoids. Immunology. 1999 Sep;98(1):104-10.
PubMed
Brattig NW, Lepping B, Timmann C, Büttner DW, Marfo Y, Hamelmann C, Horstmann RD: Onchocerca volvulus-exposed persons fail to produce interferon-gamma in response to O. volvulus antigen but mount proliferative responses with interleukin-5 and IL-13 production that decrease with increasing microfilarial density. J Infect Dis. 2002 Apr 15;185(8):1148-54.
PubMed
Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA: Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006 Jul;118(3):353-60.
PubMed
David A. Kosub, Ginger Lehrman, Jeffrey M. Milush, Dejiang Zhou, Elizabeth Chacko, Amanda Leone, Shari Gordon, Guido Silvestri, James G. Else, Philip Keiser, Mamta K. Jain, Donald L. Sodora: Gamma/Delta T-Cell Functional Responses Differ after Pathogenic Human Immunodeficiency Virus and Nonpathogenic Simian Immunodeficiency Virus Infections. J Virol. 2008 February; 82(3): 1155–1165.
PubMed
Erik L. Brincks, Tamara A. Kucaba, Kevin L. Legge, Thomas S. Griffith: Influenza-induced expression of functional TNF-related apoptosis-inducing ligand (TRAIL) on human PBMC. Hum Immunol. 2008 October; 69(10): 634–646.
PubMed
Variant
100 tests
1F-706-T100
In stock
25 tests
1F-706-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock
0.1 mg
11-261-C100
In stock
Contact us
Datasheet download
Bulk requests
Technical support for this product