Anti-IFN gamma Biotin

Anti-IFN gamma Biotin
Regulatory status
RUO
Antigen
IFN gamma
Clone
4S.B3
Format
Biotin
Reactivity
Non-human primates, Human
Variant
0.1 mg
1B-706-C100
In stock
198.00 USD

0.025 mg
1B-706-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
1B-706-C100
In stock
198.00 USD

0.025 mg
1B-706-C025
Delivery 1 week
99.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 4S.B3 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein.
Application details
Flow cytometry: Recommended dilution 1-4 μg/ml. Intracellular staining.
ELISA: Detection antibody in combination with capture antibody NIB42.
Reactivity
Non-human primates, Human
Immunogen
Interferon gamma derived from human leukocytes
Concentration
1 mg/ml
Preparation
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Interferon gamma, IFN-gamma
Antigen description
The interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.
Entrez Gene ID 3458
UniProt ID P01579
1B-706_FC_Profil Značení
Flow cytometry intracellular staining pattern of human peripheral whole blood (PHA stimulated and Brefeldin A + Monesin treated) stained using anti-human IFN-gamma (4S.B3) biotin antibody (concentration in sample 1,7 μg/ml, Streptavidin APC).
1B-706_ELISA
Cytokine IFN gamma (IFNƴ) sandwich ELISA (enzyme-linked immunosorbent assay) that utilizes antibody clone NIB42 as the protein capture antibody and antibody clone 4S.B3-Biotin as the detection antibody. Recombinant IFNƴ was captured by NIB42 and detected by variable amounts of 4S.B3-Biotin. The 4S.B3-Biotin conjugate was visualized by Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of cytokine and to the amount of the detection antibody.
1B-706_FC_Dot-plot
Flow cytometry multicolor intracellular staining pattern of human lymphocytes (PHA stimulated and Brefeldin A + Monesin treated) stained using anti-human IFN-gamma (4S.B3) biotin antibody (concentration in sample 1,7 μg/ml, Streptavidin APC) and anti-human CD3 (UCHT1) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).
1B-706_FC_Histogram
Separation of human IFN-gamma positive CD3 positive lymphocytes (red-filled) from IFN-gamma negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood (PHA stimulated and Brefeldin A + Monesin treated) stained using anti-human IFN-gamma (4S.B3) biotin antibody (concentration in sample 1,7 μg/ml, Streptavidin APC).

General references:

Schroder K, Hertzog PJ, Ravasi T, Hume DA: Interferon-gamma: an overview of signals, mechanisms and functions. J Leukoc Biol. 2004 Feb;75(2):163-89.
PubMed
Ellis TN, Beaman BL: Interferon-gamma activation of polymorphonuclear neutrophil function. Immunology. 2004 May;112(1):2-12.
PubMed
Costa-Pereira AP, Williams TM, Strobl B, Watling D, Briscoe J, Kerr IM: The antiviral response to gamma interferon. J Virol. 2002 Sep;76(18):9060-8.
PubMed
Okamura H, Kashiwamura S, Tsutsui H, Yoshimoto T, Nakanishi K: Regulation of interferon-gamma production by IL-12 and IL-18. Curr Opin Immunol. 1998 Jun;10(3):259-64.
PubMed
Young HA, Hardy KJ: Role of interferon-gamma in immune cell regulation. J Leukoc Biol. 1995 Oct;58(4):373-81. Review.
PubMed

Product specific references:

Coles AJ, Wing M, Smith S, Coraddu F, Greer S, Taylor C, Weetman A, Hale G, Chatterjee VK, Waldmann H, Compston A: Pulsed monoclonal antibody treatment and autoimmune thyroid disease in multiple sclerosis. Lancet. 1999 Nov 13;354(9191):1691-5.
PubMed
Meager A, Parti S, Barwick S, Spragg J, O'Hagan K: Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon. J Interferon Res. 1984 Fall;4(4):619-25.
PubMed
Caulfield JJ, Fernandez MH, Sousa AR, Lane SJ, Lee TH, Hawrylowicz CM: Regulation of major histocompatibility complex class II antigens on human alveolar macrophages by granulocyte-macrophage colony-stimulating factor in the presence of glucocorticoids. Immunology. 1999 Sep;98(1):104-10.
PubMed
Brattig NW, Lepping B, Timmann C, Büttner DW, Marfo Y, Hamelmann C, Horstmann RD: Onchocerca volvulus-exposed persons fail to produce interferon-gamma in response to O. volvulus antigen but mount proliferative responses with interleukin-5 and IL-13 production that decrease with increasing microfilarial density. J Infect Dis. 2002 Apr 15;185(8):1148-54.
PubMed
Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA: Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006 Jul;118(3):353-60.
PubMed
David A. Kosub, Ginger Lehrman, Jeffrey M. Milush, Dejiang Zhou, Elizabeth Chacko, Amanda Leone, Shari Gordon, Guido Silvestri, James G. Else, Philip Keiser, Mamta K. Jain, Donald L. Sodora: Gamma/Delta T-Cell Functional Responses Differ after Pathogenic Human Immunodeficiency Virus and Nonpathogenic Simian Immunodeficiency Virus Infections. J Virol. 2008 February; 82(3): 1155–1165.
PubMed
Erik L. Brincks, Tamara A. Kucaba, Kevin L. Legge, Thomas S. Griffith: Influenza-induced expression of functional TNF-related apoptosis-inducing ligand (TRAIL) on human PBMC. Hum Immunol. 2008 October; 69(10): 634–646.
PubMed
Variant
0.1 mg
1B-706-C100
In stock
198.00 USD

0.025 mg
1B-706-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
1B-706-C100
In stock
198.00 USD

0.025 mg
1B-706-C025
Delivery 1 week
99.00 USD

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