Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu IL-2 Purified

Anti-Hu IL-2 Purified

Regulatory status

RUO

Antigen

IL-2

Clone

35C3

Format

Purified

Reactivity

Human

Application

ELISA, IP, WB, FC (QC tested), ICC

Variant

0.1 mg

11-936-C100

In stock

154.00 USD

Variant

0.1 mg

11-936-C100

In stock

154.00 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody 35C3 recognizes human interleukin 2 (IL-2; secreted or intracellular).

Application

ELISA, IP, WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 0.5-4 µg/ml. Intracellular staining.

Reactivity

Human

Immunogen

recombinant human IL-2

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Interleukin 2

Antigen description

IL-2 (interleukin 2) is a cytokine that is produced primarily by stimulated Th cells and its crucial role is induction of T cell proliferation. However, IL-2 also stimulates growth and differentiation of B cells, NK cells, monocytes and other cell types, such as LAK cells or oligodendrocytes and is one of the key molecules of the immune system. IL-2 signaling pathways lead to induction of Bcl-2 protein.

Entrez Gene ID 3558

UniProt ID P60568

Flow cytometry intracellular staining pattern of human PMA + ionomycin stimulated and Brefeldin A treated peripheral whole blood stained using anti-human IL-2 (35C3) purified antibody (concentration in sample 0,5 μg/ml, GAM APC).

Flow cytometry multicolor intracellular staining of human PMA + ionomycin stimulated and Brefeldin A treated peripheral whole blood showing lymphocytes stained using anti-human CD4 (MEM-241) PE-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human IL-2 (35C3) purified antibody (concentration in sample 0,5 μg/ml, GAM APC).

Separation of human CD4 positive IL-2 positive lymphocytes (red-filled) from CD4 negative IL-2 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human PMA + ionomycin stimulated and Brefeldin A treated peripheral whole blood stained using anti-human IL-2 (35C3) purified antibody (concentration in sample 0,5 μg/ml, GAM APC).