Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu IL-2 PE-Cy™7

Anti-Hu IL-2 PE-Cy™7

Regulatory status

RUO

Antigen

IL-2

Clone

35C3

Format

PE-Cy™7

Reactivity

Human

Application

FC-IC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

T7-936-T100

In stock

282.70 USD

Variant

100 tests

T7-936-T100

In stock

282.70 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody 35C3 recognizes human interleukin 2 (IL-2; secreted or intracellular).

Application

FC-IC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.

Reactivity

Human

Immunogen

recombinant human IL-2

Preparation

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

Interleukin 2

Antigen description

IL-2 (interleukin 2) is a cytokine that is produced primarily by stimulated Th cells and its crucial role is induction of T cell proliferation. However, IL-2 also stimulates growth and differentiation of B cells, NK cells, monocytes and other cell types, such as LAK cells or oligodendrocytes and is one of the key molecules of the immune system. IL-2 signaling pathways lead to induction of Bcl-2 protein.

Entrez Gene ID 3558

UniProt ID P60568

Flow cytometry intracellular staining pattern of human stimulated (PMA + Ionomycin) and brefeldin treated peripheral whole blood stained using anti-human IL-2 (35C3) PE-Cy™7 antibody (concentration in sample 3 μg/ml).

Flow cytometry multicolor surface staining pattern of human stimulated (PMA + Ionomycin) and brefeldin treated lymphocytes stained using anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and intracellularly stained by anti-human IL-2 (35C3) PE-Cy™7 antibody (concentration in sample 3 μg/ml).

Separation of human CD3 positive IL-2 positive T cells (red-filled) from CD3 negative IL-2 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human stimulated (PMA + Ionomycin) and brefeldin treated peripheral whole blood stained using anti-human IL-2 (35C3) PE-Cy™7 antibody (concentration in sample 3 μg/ml).