Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu IL-2 Biotin

Anti-Hu IL-2 Biotin

Regulatory status

RUO

Antigen

IL-2

Clone

35C3

Format

Biotin

Reactivity

Human

Application

ELISA, IP, WB, FC (QC tested), ICC

Variant

0.1 mg

1B-936-C100

In stock

198.00 USD

Variant

0.1 mg

1B-936-C100

In stock

198.00 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody 35C3 recognizes human interleukin 2 (IL-2; secreted or intracellular).

Application

ELISA, IP, WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 0.5-4 μg/ml.

Reactivity

Human

Immunogen

recombinant human IL-2

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Interleukin 2

Antigen description

IL-2 (interleukin 2) is a cytokine that is produced primarily by stimulated Th cells and its crucial role is induction of T cell proliferation. However, IL-2 also stimulates growth and differentiation of B cells, NK cells, monocytes and other cell types, such as LAK cells or oligodendrocytes and is one of the key molecules of the immune system. IL-2 signaling pathways lead to induction of Bcl-2 protein.

Entrez Gene ID 3558

UniProt ID P60568

ELISA analysis of human IL-2 (100 ng/ml) using biotin-conjugated mouse monoclonal antibody 35C3 in five different concentrations (0.0016 - 1 μg/ml).

Flow cytometry multicolor surface staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human lymphocytes using anti-human CD3 (UCHT1) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood) and intracellular staining of human lymphocytes using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5 μg/ml, Streptavidin PE).

Flow cytometry multicolor intracellular staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human lymphocytes using anti-human CD4 (MEM-241) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and intracellular staining of human lymphocytes using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5 μg/ml, Streptavidin PE).

Separation of human CD3 positive IL-2 positive lymphocytes (red-filled) from CD3 negative IL-2 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5 μg/ml, Streptavidin PE).

Flow cytometry intracellular staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5 μg/ml, Streptavidin PE).