Anti-Hu IL-17A APC

Anti-Hu IL-17A APC
Regulatory status
RUO
Antigen
IL-17A
Clone
9F9
Format
APC
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-937-T100
In stock
242.00 USD
Variant
100 tests
1A-937-T100
In stock
242.00 USD
Product details
Description
Images
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 9F9 recognizes human interleukin 17A (IL-17A; secreted or intracellular).
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
Reactivity
Human
Immunogen
mammalian-derived human IL-17-IgG fusion protein, boost with recombinant human IL-17A
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Interleukin 17
Antigen description
Interleukin 17A (IL-17A) is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins. It plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. It is the signature effector cytokine of Th17 cells, and in this role it primarily induces neutrophil activation and recruitment at infection and inflammatory sites. High levels of IL-17A are associated with rheumatoid arthritis, psoriasis, multiple sclerosis, and another inflammatory diseases, including lung injugy during severe COVID 19. This cytokine also contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. It is an effector cytokine for invariant NKT cells (iNKT), and it is involved in epithelial barrier formation upon injury.
Entrez Gene ID 3605
UniProt ID Q16552
1A-937_FC_Profil Značení
Flow cytometry intracellular staining pattern of PHA stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-17A (9F9) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1A-937_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of PHA stimulated and Brefeldin A treated human lymphocytes using anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and intracellular staining using anti-human IL-17A (9F9) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1A-937_FC_Histogram
Separation of human CD17A positive CD3 positive lymphocytes (red-filled) from CD17A negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PHA stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-17A (9F9) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
Variant
100 tests
1A-937-T100
In stock
242.00 USD
Variant
100 tests
1A-937-T100
In stock
242.00 USD

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