Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu IL-17A Alexa Fluor<sup>®</sup> 647

Anti-Hu IL-17A Alexa Fluor^® 647

Regulatory status

RUO

Antigen

IL-17A

Clone

9F9

Format

Alexa Fluor^® 647

Reactivity

Human

Application

FC-IC (QC tested), ICC

Excitation laser

red (633 nm)

Variant

100 tests

A6-937-T100

In stock

242.00 USD

Variant

100 tests

A6-937-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 9F9 recognizes human interleukin 17A (IL-17A; secreted or intracellular).

Application

FC-IC (QC tested), ICC

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.

Reactivity

Human

Immunogen

mammalian-derived human IL-17-IgG fusion protein, boost with recombinant human IL-17A

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

Interleukin 17

Antigen description

Interleukin 17A (IL-17A) is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins. It plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. It is the signature effector cytokine of Th17 cells, and in this role it primarily induces neutrophil activation and recruitment at infection and inflammatory sites. High levels of IL-17A are associated with rheumatoid arthritis, psoriasis, multiple sclerosis, and another inflammatory diseases, including lung injugy during severe COVID 19. This cytokine also contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. It is an effector cytokine for invariant NKT cells (iNKT), and it is involved in epithelial barrier formation upon injury.

Entrez Gene ID 3605

UniProt ID Q16552

Flow cytometry multicolor surface staining pattern of human PHA stimulated and brefeldin treated lymphocytes stained using anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human IL-17A (9F9) Alexa Fluor® 647 antibody (concentration in sample 1.67 μg/ml).

Separation of human CD3 positive IL-17A positive T cells (red-filled) from CD3 negative IL-17A negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated and brefeldin treated peripheral blood mononuclear cells stained using anti-human IL-17A (9F9) Alexa Fluor® 647 antibody (concentration in sample 1.67 μg/ml).