Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu IgM HRP

Anti-Hu IgM HRP

Regulatory status

RUO

Antigen

IgM

Clone

CH2

Format

HRP

Reactivity

Human

Application

ELISA, WB

Variant

0.1 mg

1X-320-C100

In stock

209.00 USD

0.025 mg

1X-320-C025

Delivery 1 week

104.50 USD

Variant

0.1 mg

1X-320-C100

In stock

209.00 USD

0.025 mg

1X-320-C025

Delivery 1 week

104.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody CH2 reacts with Fc fragment of human IgM.

Application

ELISA, WB

Application details

Western blotting: Recommended dilution: 1 μg/ml.
Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining.

Reactivity

Human

Immunogen

Purified human IgM.

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01% thimerosal

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

immunoglobulin M

Antigen description

Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.

Western blotting detection (reducing conditions) of IgM in human plasma using anti-human IgM (CH2) peroxidase conjugate.

ELISA analysis of IgM in human serum using capture antibody MA2 and HRP-conjugated detection antibody CH2. Three different dilutions of human serum were used, and 10% BSA as negative control.

General references:

Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed

Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71.
PubMed

Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34.
PubMed

Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32.
PubMed