Anti-Hu IgM HRP

Anti-Hu IgM HRP
Regulatory status
RUO
Antigen
IgM
Clone
CH2
Format
HRP
Reactivity
Human
Application
Variant
0.1 mg
1X-320-C100
In stock
190.00 USD

0.025 mg
1X-320-C025
Delivery 1 week
95.00 USD
Variant
0.1 mg
11-261-C100
In stock
190.00 USD

0.1 mg
11-261-C100
In stock
95.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody CH2 reacts with Fc fragment of human IgM.
Application
Application details
Western blotting: Recommended dilution: 1 μg/ml.
Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining.
Reactivity
Human
Immunogen
Purified human IgM.
Concentration
1 mg/ml
Preparation
Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01% thimerosal
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
immunoglobulin M
Antigen description
Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
1X-320 WB
Western blotting detection (reducing conditions) of IgM in human plasma using anti-human IgM (CH2) peroxidase conjugate.
1X-320_ELISA
ELISA analysis of IgM in human serum using capture antibody MA2 and HRP-conjugated detection antibody CH2. Three different dilutions of human serum were used, and 10% BSA as negative control.

General references:

Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed
Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71.
PubMed
Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34.
PubMed
Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32.
PubMed
Variant
0.1 mg
1X-320-C100
In stock
190.00 USD

0.025 mg
1X-320-C025
Delivery 1 week
95.00 USD
Variant
0.1 mg
11-261-C100
In stock
190.00 USD

0.1 mg
11-261-C100
In stock
95.00 USD