Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu IgM FITC

Anti-Hu IgM FITC

Regulatory status

RUO

Antigen

IgM

Clone

CH2

Format

FITC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1F-320-C100

In stock

165.00 USD

0.025 mg

1F-320-C025

Delivery 1 week

82.50 USD

Variant

0.1 mg

1F-320-C100

In stock

165.00 USD

0.025 mg

1F-320-C025

Delivery 1 week

82.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody CH2 reacts with Fc fragment of human IgM.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml. Extracellular and intracellular staining.

Reactivity

Human

Immunogen

Purified human IgM.

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

immunoglobulin M

Antigen description

Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgM (CH2) FITC antibody (concentration in sample 1 μg/ml).

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human IgM (CH2) FITC antibody (concentration in sample 1 μg/ml) and anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor staining pattern of human lymphocytes using anti-human CD19 (LT19) PE-Cy™7 antibody (surface staining, 4 μl reagent / 100 μl of peripheral whole blood) anti-human IgM (CH2) FITC antibody (intracellular staining, concentration in sample 3 μg/ml) after blocking of surface IgM using anti-human IgM (CH2) APC antibody (surface staining, concentration in sample 10 μg/ml).

Separation of human IgM positive CD19 positive B-cells (red-filled) from IgM negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgM (CH2) FITC antibody (concentration in sample 1 μg/ml).

General references:

Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed

Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71.
PubMed

Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34.
PubMed

Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32.
PubMed