Anti-Hu IgM FITC

Anti-Hu IgM FITC
Regulatory status
RUO
Antigen
IgM
Clone
CH2
Format
FITC
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1F-320-C100
In stock
150.00 USD

0.025 mg
1F-320-C025
In stock
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD

0.1 mg
11-261-C100
In stock
75.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody CH2 reacts with Fc fragment of human IgM.
Application
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml. Extracellular and intracellular staining.
Reactivity
Human
Immunogen
Purified human IgM.
Concentration
1 mg/ml
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
immunoglobulin M
Antigen description
Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
1F-320_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgM (CH2) FITC antibody (concentration in sample 1 μg/ml).
1F-320_FC_Dot-Plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human IgM (CH2) FITC antibody (concentration in sample 1 μg/ml) and anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1F-320_FC_Dot-plot_2
Flow cytometry multicolor staining pattern of human lymphocytes using anti-human CD19 (LT19) PE-Cy™7 antibody (surface staining, 4 μl reagent / 100 μl of peripheral whole blood) anti-human IgM (CH2) FITC antibody (intracellular staining, concentration in sample 3 μg/ml) after blocking of surface IgM using anti-human IgM (CH2) APC antibody (surface staining, concentration in sample 10 μg/ml).
1F-320_FC_Histogram
Separation of human IgM positive CD19 positive B-cells (red-filled) from IgM negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgM (CH2) FITC antibody (concentration in sample 1 μg/ml).

General references:

Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed
Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71.
PubMed
Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34.
PubMed
Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32.
PubMed
Variant
0.1 mg
1F-320-C100
In stock
150.00 USD

0.025 mg
1F-320-C025
In stock
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD

0.1 mg
11-261-C100
In stock
75.00 USD

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