Anti-Hu IgG (Fc) Purified

Anti-Hu IgG (Fc) Purified
Regulatory status
RUO
Antigen
IgG (Fc)
Clone
EM-07
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-536-C100
In stock
100.00 USD

0.025 mg
11-536-C025
Delivery 1 week
50.00 USD
Variant
0.1 mg
11-261-C100
In stock
100.00 USD

0.1 mg
11-261-C100
In stock
50.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Human
Immunogen
Fusion protein of human IgG Fc fragment.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Immunoglobulin G Fc fragment
Antigen description
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
11-536 WB
Western blotting detection of IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purified from human serum, with MEM-09 antibody against IgG κ light chain (A) and EM-07 antibody against IgG Fc fragment (B).
11-536_FC_Dot-plot
Flow cytometry multicolor surface staining of human peripheral whole blood stained using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 μg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 μl reagent / 100 μl of peripheral whole blood).
11-536_FC_Histogram
Separation of human monocytes (red-filled) from CD3 negative IgG (Fc) positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 μg/ml, GAM APC). The pattern varies depending on the blood donor.
11-536_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 μg/ml, GAM APC). The pattern varies depending on the blood donor.
11-536_FC_Profil Značení_1
Flow cytometry surface staining pattern of human peripheral whole blood using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 μg/ml, GAM APC). The pattern varies depending on the blood donor.

General references:

Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed
Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71.
PubMed
Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34.
PubMed
Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32.
PubMed
Variant
0.1 mg
11-536-C100
In stock
100.00 USD

0.025 mg
11-536-C025
Delivery 1 week
50.00 USD
Variant
0.1 mg
11-261-C100
In stock
100.00 USD

0.1 mg
11-261-C100
In stock
50.00 USD

Related products

-134%
Reg. statusCE IVD
ApplicationFC
100 ml
200.00 USD
-134%
Reg. statusCE IVD
ApplicationFC
100 ml
60.00 USD
-134%
Reg. statusRUO
ApplicationFC
100 ml
50.00 USD
-134%
CloneMOPC-21
Reg. statusRUO
0.1 mg
105.00 USD