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Exbio
—
Research products
—
Antibodies
—
Serum, plasma, and secreted proteins
—
Anti-Hu IgG (Fc) Purified
Anti-Hu IgG (Fc) Purified
Regulatory status
RUO
Antigen
IgG (Fc)
Clone
EM-07
Format
Purified
Reactivity
Human
Application
ELISA, WB, ICC
Variant
0.1 mg
11-536-C100
In stock
100.00 USD
0.025 mg
11-536-C025
Out of stock
50.00 USD
Variant
0.1 mg
11-261-C100
In stock
100.00 USD
0.1 mg
11-261-C100
In stock
50.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Application
ELISA, WB, ICC
Application details
Western blotting and ELISA: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM.
Reactivity
Human
Immunogen
Fusion protein of human IgG Fc fragment.
Other names
Immunoglobulin G Fc fragment
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Western blotting detection of IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purified from human serum, with MEM-09 antibody against IgG κ light chain (A) and EM-07 antibody against IgG Fc fragment (B).
General references:
Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed
Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71.
PubMed
Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34.
PubMed
Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32.
PubMed
Variant
0.1 mg
11-536-C100
In stock
100.00 USD
0.025 mg
11-536-C025
Out of stock
50.00 USD
Variant
0.1 mg
11-261-C100
In stock
100.00 USD
0.1 mg
11-261-C100
In stock
50.00 USD
Contact distributor
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