Anti-Hu IgG (Fc) HRP

Anti-Hu IgG (Fc) HRP
Regulatory status
RUO
Antigen
IgG (Fc)
Clone
EM-07
Format
HRP
Reactivity
Human
Application
Variant
0.1 mg
1X-536-C100
In stock
209.00 USD

0.025 mg
1X-536-C025
Delivery 1 week
104.50 USD
Variant
0.1 mg
1X-536-C100
In stock
209.00 USD

0.025 mg
1X-536-C025
Delivery 1 week
104.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Application
Application details
Western blotting and ELISA: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM.
Reactivity
Human
Immunogen
Fusion protein of human IgG Fc fragment.
Concentration
1 mg/ml
Preparation
Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01% thimerosal
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Immunoglobulin G Fc fragment
Antigen description
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
1X-536 ELISA
ELISA detection of IgG in human serum using HRP conjugated EM-07 antibody against IgG Fc fragment.

General references:

Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed
Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71.
PubMed
Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34.
PubMed
Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32.
PubMed
Variant
0.1 mg
1X-536-C100
In stock
209.00 USD

0.025 mg
1X-536-C025
Delivery 1 week
104.50 USD
Variant
0.1 mg
1X-536-C100
In stock
209.00 USD

0.025 mg
1X-536-C025
Delivery 1 week
104.50 USD