Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu IgG (Fab) PE-Cy™5

Anti-Hu IgG (Fab) PE-Cy™5

Regulatory status

RUO

Antigen

IgG (Fab)

Clone

4A11

Format

PE-Cy™5

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

T8-319-T100

Ask for delivery term HERE

190.00 USD

25 tests

T8-319-T025

Delivery 1 week

95.00 USD

Variant

100 tests

T8-319-T100

Ask for delivery term HERE

190.00 USD

25 tests

T8-319-T025

Delivery 1 week

95.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG2b

Specificity

The mouse monoclonal antibody 4A11 reacts with Fab fragment of human IgG. This antibody detects Fab fragments regardless light chain type.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Purified human IgG.

Preparation

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 5 (PE-Cy™5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

Immunoglobulin G Fab fragment

Antigen description

Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.

Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human IgG Fab fragment (4A11) PE-Cy™5.

General references:

Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32.
PubMed

Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34.
PubMed

Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71.
PubMed

Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed

Variant

100 tests

T8-319-T100

Ask for delivery term HERE

190.00 USD

25 tests

T8-319-T025

Delivery 1 week

95.00 USD

Variant