Anti-Hu IgG (Fab) PE-Cy™5

Anti-Hu IgG (Fab) PE-Cy™5
Regulatory status
RUO
Antigen
IgG (Fab)
Clone
4A11
Format
PE-Cy™5
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
T8-319-T100
In stock
209.00 USD

25 tests
T8-319-T025
Delivery 1 week
104.50 USD
Variant
100 tests
T8-319-T100
In stock
209.00 USD

25 tests
T8-319-T025
Delivery 1 week
104.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2b
Specificity
The mouse monoclonal antibody 4A11 reacts with Fab fragment of human IgG. This antibody detects Fab fragments regardless light chain type.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Purified human IgG.
Preparation
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 5 (PE-Cy™5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
Immunoglobulin G Fab fragment
Antigen description
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
T8-319 FC
Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human IgG Fab fragment (4A11) PE-Cy™5.

General references:

Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32.
PubMed
Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34.
PubMed
Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71.
PubMed
Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed
Variant
100 tests
T8-319-T100
In stock
209.00 USD

25 tests
T8-319-T025
Delivery 1 week
104.50 USD
Variant
100 tests
T8-319-T100
In stock
209.00 USD

25 tests
T8-319-T025
Delivery 1 week
104.50 USD

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