Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu IgE Biotin

Anti-Hu IgE Biotin

Anti-Hu IgE Biotin
Regulatory status
RUO
Antigen
IgE
Clone
BE5
Format
Biotin
Reactivity
Human
Application
ELISA
Variant
0.1 mg
1B-324-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody BE5 reacts with human IgE; it recognizes an epitope different from the ones recognized by 4G7 and 4H10 antibodies to IgE.
Application
ELISA
Application details
Flow cytometry: Recommended dilution: 1-2 μg/ml.
Reactivity
Human
Immunogen
Purified human IgE.
Other names
Immunoglobulin E
Concentration
1 mg/ml
Preparation
The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1B-324_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgE (BE5) Biotin antibody (concentration in sample 4 μg/ml) Streptavidin APC.
1B-324_FC_Histogram
Separation of human IgE positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgE (BE5) Biotin antibody (concentration in sample 4 μg/ml) Streptavidin APC.

General references:

Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed
Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71.
PubMed
Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34.
PubMed
Gould HJ, Beavil RL, Vercelli D: IgE isotype determination: epsilon-germline gene transcription, DNA recombination and B-cell differentiation. Br Med Bull. 2000;56(4):908-24.
PubMed
Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32.
PubMed

Product specific references:

Kaulfürst-Soboll H, Mertens M, Brehler R, von Schaewen A: Reduction of cross-reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis. PLoS One. 2011 Mar 14;6(3):e17800.
PubMed
Variant
0.1 mg
1B-324-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Contact distributor
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