Anti-Hu IgD Purified

Anti-Hu IgD Purified
Regulatory status
RUO
Antigen
IgD
Clone
IA6-2
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-142-C100
In stock
100.00 USD

0.025 mg
11-142-C025
Delivery 1 week
50.00 USD
Variant
0.1 mg
11-261-C100
In stock
100.00 USD

0.1 mg
11-261-C100
In stock
50.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody IA6-2 recognizes human immunoglobulin D.
Application
Application details
Flow cytometry: Recommended dilution: 2-4 μg/ml.
Reactivity
Human
Immunogen
Human IgD
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Immunoglobulin D
Antigen description
Immunoglobulin D (IgD) is expressed on the surface of naive mature B cells, thus later than IgM, and is coexpressed with it then. Triggered by antigen binding, it signals through the CD79 complex to activate the B cells. Expression of IgD is lost after the isotype switch. Soluble IgD is present in very small amounts in the serum. IgD can bind to basophils and mast cells to activate them in an IgE-independent way to participate in respiratory immune defense.
11-142_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgD (IA6-2) purified antibody (concentration in sample 0,33 μg/ml, GAM APC).
11-142_FC_Histogram
Separation of human IgD positive lymphocytes (red-filled) from neutrofil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human IgD (IA6-2) purified antibody (concentration in sample 0,33 μg/ml, GAM APC).

Product specific references:

Bunch DO, McGregor JG, Khandoobhai NB, Aybar LT, Burkart ME, Hu Y, Hogan SL, Poulton CJ, Berg EA, Falk RJ, Nachman PH: Decreased CD5⁺ B cells in active ANCA vasculitis and relapse after rituximab. Clin J Am Soc Nephrol. 2013 Mar;8(3):382-91
PubMed
Baier G, Baier-Bitterlich G, Looney DJ, Altman A: Immunogenic targeting of recombinant peptide vaccines to human antigen-presenting cells by chimeric anti-HLA-DR and anti-surface immunoglobulin D antibody Fab fragments in vitro. J Virol. 1995 Apr;69(4):2357-65.
PubMed
Ferrari S, Giliani S, Insalaco A, Al-Ghonaium A, Soresina AR, Loubser M, Avanzini MA, Marconi M, Badolato R, Ugazio AG, Levy Y, Catalan N, Durandy A, Tbakhi A, Notarangelo LD, Plebani A: Mutations of CD40 gene cause an autosomal recessive form of immunodeficiency with hyper IgM. Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12614-9.
PubMed
Di Sabatino A, Carsetti R, Rosado MM, Ciccocioppo R, Cazzola P, Morera R, Tinozzi FP, Tinozzi S, Corazza GR: Immunoglobulin M memory B cell decrease in inflammatory bowel disease. Eur Rev Med Pharmacol Sci. 2004 Sep-Oct;8(5):199-203.
PubMed
Pugh-Bernard AE, Silverman GJ, Cappione AJ, Villano ME, Ryan DH, Insel RA, Sanz I: Regulation of inherently autoreactive VH4-34 B cells in the maintenance of human B cell tolerance. J Clin Invest. 2001 Oct;108(7):1061-70.
PubMed
Degauque N, Ngono AE, Akl A, Lepetit M, Crochette R, Giral M, Lepourry J, Pallier A, Castagnet S, Dugast E, Guillot-Gueguen C, Jacq-Foucher M, Saulquin X, Cesbron A, Laplaud D, Nicot A, Brouard S, Soulillou JP: Characterization of antigen-specific B cells using nominal antigen-coated flow-beads. PLoS One. 2013 Dec 30;8(12):e84273.
PubMed
Chang NH, McKenzie T, Bonventi G, Landolt-Marticorena C, Fortin PR, Gladman D, Urowitz M, Wither JE: Expanded population of activated antigen-engaged cells within the naive B cell compartment of patients with systemic lupus erythematosus. J Immunol. 2008 Jan 15;180(2):1276-84.
PubMed
Variant
0.1 mg
11-142-C100
In stock
100.00 USD

0.025 mg
11-142-C025
Delivery 1 week
50.00 USD
Variant
0.1 mg
11-261-C100
In stock
100.00 USD

0.1 mg
11-261-C100
In stock
50.00 USD

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