Anti-Hu Ig Lambda Light Chain PE

Anti-Hu Ig Lambda Light Chain PE
Regulatory status
RUO
Antigen
Ig Lambda Light Chain
Clone
4C2
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-505-T100
In stock
308.00 USD

25 tests
1P-505-T025
Delivery 1 week
154.00 USD
Variant
100 tests
1P-505-T100
In stock
308.00 USD

25 tests
1P-505-T025
Delivery 1 week
154.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody 4C2 reacts with lambda light chains (22.5 kDa) of human immunoglobulin.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human
Negative species
Rabbit, Hamster, Sheep, Guinea pig, Goat
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Immunoglobulin lambda, Igl
Antigen description
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
1P-505_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Ig Lambda Light Chain (4C2) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).
1P-505_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human Ig Lambda Light Chain (4C2) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).
1P-505_FC_Histogram
Separation of human CD19 positive Lambda positive B cells (red-filled) from CD19 negative Lambda negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Ig Lambda Light Chain (4C2) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).
1P-505 FC
Flow cytometry analysis of B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody.

General references:

Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed

Product specific references:

Os A, Bürgler S, Ribes AP, Funderud A, Wang D, Thompson KM, Tjønnfjord GE, Bogen B, Munthe LA: Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells. Cell Rep. 2013 Aug 15;4(3):566-77.
PubMed
Variant
100 tests
1P-505-T100
In stock
308.00 USD

25 tests
1P-505-T025
Delivery 1 week
154.00 USD
Variant
100 tests
1P-505-T100
In stock
308.00 USD

25 tests
1P-505-T025
Delivery 1 week
154.00 USD

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