Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu Ig Lambda Light Chain APC

Anti-Hu Ig Lambda Light Chain APC

Anti-Hu Ig Lambda Light Chain APC
Regulatory status
RUO
Antigen
Ig Lambda Light Chain
Clone
4C2
Format
APC
Reactivity
Human
Application
FC (QC tested)
Excitation laser
red (633 nm)
Variant
100 tests
1A-505-T100
In stock
25 tests
1A-505-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock
0.1 mg
11-261-C100
In stock
Contact us
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody 4C2 reacts with lambda light chains (22.5 kDa) of human immunoglobulin.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Negative species
Rabbit, Hamster, Sheep, Guinea pig, Goat
Other names
Immunoglobulin lambda, Igl
Preparation
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
1A-505_Profil
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Ig Lambda Light Chain (4C2) APC (10 μl reagent / 100 μl of peripheral whole blood).
1A-505_Histogram
Separation of human Ig Lambda Light Chain positive B-Lymphocytes (red-filled) from human Ig Lambda Light Chain negative B-Lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Ig Lambda Light Chain (4C2) APC (10 μl reagent / 100 μl of peripheral whole blood).

General references:

Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed

Product specific references:

Os A, Bürgler S, Ribes AP, Funderud A, Wang D, Thompson KM, Tjønnfjord GE, Bogen B, Munthe LA: Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells. Cell Rep. 2013 Aug 15;4(3):566-77.
PubMed
Variant
100 tests
1A-505-T100
In stock
25 tests
1A-505-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock
0.1 mg
11-261-C100
In stock
Contact us
Datasheet download
Bulk requests
Technical support for this product