Anti-Hu Ig Lambda Light Chain Alexa Fluor® 488

Anti-Hu Ig Lambda Light Chain Alexa Fluor<sup>®</sup> 488
Regulatory status
RUO
Antigen
Ig Lambda Light Chain
Clone
1-155-2
Format
Alexa Fluor® 488
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
A4-673-T100
In stock
242.00 USD
Variant
100 tests
A4-673-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 1-155-2 recognizes lambda light chains (22.5 kDa) of human immunoglobulin.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
Reactivity
Human
Immunogen
Human IgA1 lambda myeloma protein
Preparation
Purified antibody is conjugated with Alexa Fluor® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
Immunoglobulin lambda, Igl
Antigen description
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
A4-673_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Lambda Light Chain (1-155-2) Alexa Fluor® 488 antibody (4 μl reagent / 100 μl of peripheral whole blood).
A4-673_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human Lambda Light Chain (1-155-2) Alexa Fluor® 488 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
A4-673_FC_Histogram
Separation of human Lambda Light Chain positive B cells (red-filled) from Lambda Light Chain negative B cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Lambda Light Chain (1-155-2) Alexa Fluor® 488 antibody (4 μl reagent / 100 μl of peripheral whole blood).

General references:

Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed

Product specific references:

Kubagawa H, Gathings WE, Levitt D, Kearney JF, Cooper MD: Immunoglobulin isotype expression of normal pre-B cells as determined by immunofluorescence. J Clin Immunol. 1982 Oct;2(4):264-9.
PubMed
Bahler DW, Pindzola JA, Swerdlow SH: Splenic marginal zone lymphomas appear to originate from different B cell types. Am J Pathol. 2002 Jul;161(1):81-8.
PubMed
Tierens AM, Holte H, Warsame A, Ikonomou IM, Wang J, Chan WC, Delabie J: Low levels of monoclonal small B cells in the bone marrow of patients with diffuse large B-cell lymphoma of activated B-cell type but not of germinal center B-cell type. Haematologica. 2010 Aug;95(8):1334-41.
PubMed
Variant
100 tests
A4-673-T100
In stock
242.00 USD
Variant
100 tests
A4-673-T100
In stock
242.00 USD

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