Anti-Hu Ig Kappa Light Chain Purified

Anti-Hu Ig Kappa Light Chain Purified
Regulatory status
RUO
Antigen
Ig Kappa Light Chain
Clone
TB28-2
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-674-C100
In stock
143.00 USD

0.025 mg
11-674-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-674-C100
In stock
143.00 USD

0.025 mg
11-674-C025
Delivery 1 week
71.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The antibody TB28-2 recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml. Extracellular and intracellular staining.
Reactivity
Human
Immunogen
Human IgG-kappa myeloma protein
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Immunoglobulin kappa, Igk
Antigen description
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
11-674_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Ig Kappa Light Chain (TB28-2) purified antibody (concentration in sample 0.5 μg/ml, GAM APC).
11-674_FC_Histogram
Separation of human Ig Kappa light chain positive lymphocytes (red-filled) from Ig Kappa light chain negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Ig Kappa Light Chain (TB28-2) purified antibody (concentration in sample 0.5 μg/ml, GAM APC).

General references:

Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed

Product specific references:

Kiyotaki M, Cooper MD, Bertoli LF, Kearney JF, Kubagawa H: Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells. J Immunol. 1987 Jun 15;138(12):4150-8.
PubMed
Nakamura T, Kubagawa H, Cooper MD: Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8522-6.
PubMed
Böttcher S, Ritgen M, Buske S, Gesk S, Klapper W, Hoster E, Hiddemann W, Unterhalt M, Dreyling M, Siebert R, Kneba M, Pott C; EU MCL MRD Group: Minimal residual disease detection in mantle cell lymphoma: methods and significance of four-color flow cytometry compared to consensus IGH-polymerase chain reaction at initial staging and for follow-up examinations. Haematologica. 2008 Apr;93(4):551-9.
PubMed
Karandikar NJ, Aquino DB, McKenna RW, Kroft SH: Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome. An immunophenotypic analysis. Am J Clin Pathol. 2001 Aug;116(2):204-10.
PubMed
Jourdan M, Caraux A, De Vos J, Fiol G, Larroque M, Cognot C, Bret C, Duperray C, Hose D, Klein B: An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization. Blood. 2009 Dec 10;114(25):5173-81.
PubMed
Variant
0.1 mg
11-674-C100
In stock
143.00 USD

0.025 mg
11-674-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-674-C100
In stock
143.00 USD

0.025 mg
11-674-C025
Delivery 1 week
71.50 USD

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