Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu Ig Kappa Light Chain Purified

Anti-Hu Ig Kappa Light Chain Purified

Regulatory status

RUO

Antigen

Ig Kappa Light Chain

Clone

MEM-09

Format

Purified

Reactivity

Human

Application

ELISA, IP, WB, FC (QC tested), IHC(P), ICC

Variant

0.1 mg

11-244-C100

In stock

143.00 USD

0.025 mg

11-244-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-244-C100

In stock

143.00 USD

0.025 mg

11-244-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with kappa light chains (22.5 kDa). Material immunoprecipitated from human serum with the antibody MEM-09 consists of IgG and traces of IgM.

Application

ELISA, IP, WB, FC (QC tested), IHC(P), ICC

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml.

Reactivity

Human

Immunogen

Crude thymus membrane fraction.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Immunoglobulin kappa, Igk

Antigen description

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.

Western blotting analysis (reducing conditions) of human blood sera using anti-human Immunoglobulin kappa-chain (MEM-09). Lane 1-3: Human blood serum of different healthy donors

Separation of human Ig Kappa Light Chain positive lymphocytes (red-filled) from Ig Kappa Light Chain negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Ig Kappa Light Chain (MEM-09) purified antibody (concentration in sample 3 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Ig Kappa Light Chain (MEM-09) purified antibody (concentration in sample 3 μg/ml) GAM APC.

General references:

Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed

Product specific references:

Horejsi V, Hilgert I, Kristofova H, Bazil V, Bukovsky A, Kulhankova J: Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha). 1986;32(1):12-25.
PubMed