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Exbio
—
Research products
—
Antibodies
—
Serum, plasma, and secreted proteins
—
Anti-Hu Ig Kappa Light Chain PE
Anti-Hu Ig Kappa Light Chain PE
Regulatory status
RUO
Antigen
Ig Kappa Light Chain
Clone
TB28-2
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1P-674-T100
In stock
275.00 USD
25 tests
1P-674-T025
In stock
137.50 USD
Variant
100 tests
1P-674-T100
In stock
275.00 USD
25 tests
1P-674-T025
In stock
137.50 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The antibody TB28-2 recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10
6
cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
Reactivity
Human
Immunogen
Human IgG-kappa myeloma protein
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Immunoglobulin kappa, Igk
Antigen description
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Ig Kappa Light Chain (TB28-2) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry multicolor surface staining of human CD19 positive B cells using anti-human Ig Kappa Light Chain (TB28-2) PE (c = 5 µg/ml) and anti-human Ig Lambda Light Chain (1-155-2) FITC (c = 5 µg/ml) antibodies.
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human Ig Kappa Light Chain (TB28-2) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
Separation of human Ig Lambda Kappa Chain positive B cells (red-filled) from Ig Kappa Light Chain negative B cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Ig Kappa Light Chain (TB28-2) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
General references:
Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed
Product specific references:
Kiyotaki M, Cooper MD, Bertoli LF, Kearney JF, Kubagawa H: Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells. J Immunol. 1987 Jun 15;138(12):4150-8.
PubMed
Nakamura T, Kubagawa H, Cooper MD: Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8522-6.
PubMed
Böttcher S, Ritgen M, Buske S, Gesk S, Klapper W, Hoster E, Hiddemann W, Unterhalt M, Dreyling M, Siebert R, Kneba M, Pott C; EU MCL MRD Group: Minimal residual disease detection in mantle cell lymphoma: methods and significance of four-color flow cytometry compared to consensus IGH-polymerase chain reaction at initial staging and for follow-up examinations. Haematologica. 2008 Apr;93(4):551-9.
PubMed
Karandikar NJ, Aquino DB, McKenna RW, Kroft SH: Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome. An immunophenotypic analysis. Am J Clin Pathol. 2001 Aug;116(2):204-10.
PubMed
Jourdan M, Caraux A, De Vos J, Fiol G, Larroque M, Cognot C, Bret C, Duperray C, Hose D, Klein B: An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization. Blood. 2009 Dec 10;114(25):5173-81.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
100 tests
1P-674-T100
In stock
275.00 USD
25 tests
1P-674-T025
In stock
137.50 USD
Variant
100 tests
1P-674-T100
In stock
275.00 USD
25 tests
1P-674-T025
In stock
137.50 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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