Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu Ig Kappa Light Chain FITC

Anti-Hu Ig Kappa Light Chain FITC

Regulatory status

RUO

Antigen

Ig Kappa Light Chain

Clone

A8B5

Format

FITC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1F-504-T100

In stock

252.00 USD

25 tests

1F-504-T025

Delivery 1 week

126.00 USD

Variant

100 tests

1F-504-T100

In stock

252.00 USD

25 tests

1F-504-T025

Delivery 1 week

126.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody A8B5 reacts with kappa light chains (22.5 kDa) of immunoglobulins.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Reactivity

Human

Negative species

Rabbit, Hamster, Sheep, Guinea pig, Goat

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Immunoglobulin kappa, Igk

Antigen description

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Ig kappa light chain (A8B5) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human Ig kappa light chain (A8B5) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human Ig kappa light chain positive B-lymphocytes (red-filled) from Ig kappa light chain negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Ig kappa light chain (A8B5) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry analysis of B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody.

General references:

Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
PubMed

Product specific references:

Diaz de Domingo NB, Lardo MM, Romero Artaza J, Merelli A, Carbia CD, Sánchez Avalos JC: Aplicacion de tecnicas inmunocitoquimicas para el diagnostico de neoplasias hematologicas. Medicina (B Aires). 1999;59(1):11-6.
PubMed

Scheeren FA, Diehl SA, Smit LA, Beaumont T, Naspetti M, Bende RJ, Blom B, Karube K, Ohshima K, van Noesel CJ, Spits H: IL-21 is expressed in Hodgkin lymphoma and activates STAT5: evidence that activated STAT5 is required for Hodgkin lymphomagenesis. Blood. 2008 May 1;111(9):4706-15. doi: 10.1182/blood-2007-08-105643
PubMed

Di Bartolo DL, Hyjek E, Keller S, Guasparri I, Deng H, Sun R, Chadburn A, Knowles DM, Cesarman E: Role of defective Oct-2 and OCA-B expression in immunoglobulin production and Kaposi's sarcoma-associated herpesvirus lytic reactivation in primary effusion lymphoma. J Virol. 2009 May;83(9):4308-15. doi: 10.1128/JVI.02196-08.
PubMed

Konig A, Wennemuth G, Soyer HP, Hoffmann R, Happle R, Krause W: Vulvar amyloidosis mimicking giant condylomata acuminata in a patient with multiple myeloma. Eur J Dermatol. 1999 Jan-Feb;9(1):29-31.
PubMed

Avilés-Salas A, Chanona-Vilchis JG, Candelaria-Hernández MG, Peña-Torres Mde L, Cuéllar-Mendoza ME: Presentación inicial de un linfoma plasmablástico como tumor ovárico. Ginecol Obstet Mex. 2012 Jun;80(6):430-4.
PubMed