Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu Granzyme A Purified

Anti-Hu Granzyme A Purified

Regulatory status

RUO

Antigen

Granzyme A

Clone

CB9

Format

Purified

Reactivity

Human

Application

IP, FC (QC tested), ICC

Variant

0.1 mg

11-883-C100

In stock

158.40 USD

Variant

0.1 mg

11-883-C100

In stock

158.40 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal CB9 recognizes granzyme A, a 28 kDa serine protease expressed intracellularly in activated Tc cells and NK cells.

Application

IP, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 5-10 μg/ml, intracellular staining.

Reactivity

Human

Immunogen

Full length human granzyme A

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

HFSP, CTLA3, GrA

Antigen description

Granzyme A is a serine protease expressed in the cytoplasmic granules of T cells and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Similarly to granzyme B, granzyme A acts to destroy the target cells by proteolysis of their particular components. In case of granzyme A the targets are e.g. APEX1 (it destroys its oxidative repair activity), and nucleosome assembly protein SET (it disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA).

Entrez Gene ID 3001

UniProt ID P12544

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Granzyme A (CB9) purified antibody (concentration in sample 5,0 μg/ml, GAM APC).

Separation of human Granzyme A positive NK cells (red-filled) from Granzyme A negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Granzyme A (CB9) purified antibody (concentration in sample 5,0 μg/ml, GAM APC).

Product specific references:

Choi BD, Gedeon PC, Herndon JE, Archer GE, Reap EA, Sanchez-Perez L, Mitchell DA, Bigner DD, Sampson JH: Human regulatory T cells kill tumor cells through granzyme-dependent cytotoxicity upon retargeting with a bispecific antibody. Cancer Immunol Res. 2013 Sep;1(3):163.
PubMed

Beresford PJ, Kam CM, Powers JC, Lieberman J: Recombinant human granzyme A binds to two putative HLA-associated proteins and cleaves one of them. Proc Natl Acad Sci U S A 1997 Aug 19;94(17):9285-90
PubMed

Mahrus S, Craik CS: Selective chemical functional probes of granzymes A and B reveal granzyme B is a major effector of natural killer cell-mediated lysis of target cells. Chem Biol. 2005 May;12(5):567-77.
PubMed

Bratke K, Kuepper M, Bade B, Virchow JC, Luttmann W: Differential expression of human granzymes A, B, and K in natural killer cells and during CD8+ T cell differentiation in peripheral blood. Eur J Immunol. 2005 Sep;35(9):2608-16
PubMed