Anti-Hu Granzyme A Purified

Anti-Hu Granzyme A Purified
Regulatory status
RUO
Antigen
Granzyme A
Clone
CB9
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-883-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal CB9 recognizes granzyme A, a 28 kDa serine protease expressed intracellularly in activated Tc cells and NK cells.
Application details
Flow cytometry: Recommended dilution: 5-10 μg/ml.
Reactivity
Human
Immunogen
Full length human granzyme A
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
HFSP, CTLA3, GrA
Antigen description
Granzyme A is a serine protease expressed in the cytoplasmic granules of T cells and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Similarly to granzyme B, granzyme A acts to destroy the target cells by proteolysis of their particular components. In case of granzyme A the targets are e.g. APEX1 (it destroys its oxidative repair activity), and nucleosome assembly protein SET (it disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA).
Entrez Gene ID 3001
UniProt ID P12544
11-883_FC_Profil značení
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Granzyme A (CB9) purified antibody (concentration in sample 5,0 μg/ml, GAM APC).
11-883_FC_Histogram
Separation of human Granzyme A positive NK cells (red-filled) from Granzyme A negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Granzyme A (CB9) purified antibody (concentration in sample 5,0 μg/ml, GAM APC).

Product specific references:

Choi BD, Gedeon PC, Herndon JE, Archer GE, Reap EA, Sanchez-Perez L, Mitchell DA, Bigner DD, Sampson JH: Human regulatory T cells kill tumor cells through granzyme-dependent cytotoxicity upon retargeting with a bispecific antibody. Cancer Immunol Res. 2013 Sep;1(3):163.
PubMed
Beresford PJ, Kam CM, Powers JC, Lieberman J: Recombinant human granzyme A binds to two putative HLA-associated proteins and cleaves one of them. Proc Natl Acad Sci U S A 1997 Aug 19;94(17):9285-90
PubMed
Mahrus S, Craik CS: Selective chemical functional probes of granzymes A and B reveal granzyme B is a major effector of natural killer cell-mediated lysis of target cells. Chem Biol. 2005 May;12(5):567-77.
PubMed
Bratke K, Kuepper M, Bade B, Virchow JC, Luttmann W: Differential expression of human granzymes A, B, and K in natural killer cells and during CD8+ T cell differentiation in peripheral blood. Eur J Immunol. 2005 Sep;35(9):2608-16
PubMed
Variant
0.1 mg
11-883-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD