Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu beta2-Microglobulin HRP

Anti-Hu beta2-Microglobulin HRP

Regulatory status

RUO

Antigen

beta2-Microglobulin

Clone

B2M-01

Format

HRP

Reactivity

Human

Application

ELISA, WB

Variant

0.1 mg

1X-237-C100

Ask for delivery term HERE

220.00 USD

0.025 mg

1X-237-C025

Delivery 1 week

110.00 USD

Variant

0.1 mg

1X-237-C100

Ask for delivery term HERE

220.00 USD

0.025 mg

1X-237-C025

Delivery 1 week

110.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody B2M-01 reacts with beta2-microglobulin (beta2M; extracellular antigen), associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

Application

ELISA, WB

Application details

Western blotting: Recommended dilution: 1 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes.

Reactivity

Human

Negative species

Rabbit, Dog, Mouse, Cow, Chicken

Immunogen

Purified human beta2-microglobulin

Concentration

0.2 mg/ml

Preparation

Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01% thimerosal

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

B2M

Antigen description

Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Entrez Gene ID 567

UniProt ID P61769

ELISA of HLA-G in human serum using anti-HLA-G (capture) and anti-beta2 microglobulin HRP (detection).

ELISA analysis of HLA-G and β2-microglobulin in supernatant of HLA-G transfectants with anti-β2-microglobulin (B2M-01) HRP as the detection antibody.

Product specific references:

Poláková K, Železníková T, Russ G: HLA-G5 in the blood of leukemia patients and healthy individuals. Leuk Res. 2013 Feb;37(2):139-45.
PubMed

Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA-G Expression in Human Neural Cells after Neurotropic Viral Infections. J Virol. 2005 Dec; 79(24): 15226-15237.
PubMed

Khurana M, Traum AZ, Aivado M, Wells MP, Guerrero M, Grall F, Libermann TA, Schachter AD: Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr Nephrol. 2006 Sep;21(9):1257-65. Epub 2006 Jun 30.
PubMed

Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76.
PubMed

Polakova K, Karpatova M, Russ G: Dissociation of beta 2-microglobulin is responsible for selective reduction of HLA class I antigenicity following acid treatment of cells. Mol Immunol. 1993 Oct;30(14):1223-30.
PubMed