Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu Albumin Biotin

Anti-Hu Albumin Biotin

Regulatory status

RUO

Antigen

Albumin

Clone

AL-01

Format

Biotin

Reactivity

Human

Application

ELISA, RIA, WB, IHC(P)

Variant

0.1 mg

1B-257-C100

In stock

214.50 USD

0.025 mg

1B-257-C025

Delivery 1 week

107.80 USD

Variant

0.1 mg

1B-257-C100

In stock

214.50 USD

0.025 mg

1B-257-C025

Delivery 1 week

107.80 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody AL-01 reacts only with human serum albumin, a 65-67 kDa monomeric protein in human blood plasma; it is produced in liver. No cross-reactivity was observed with other serum proteins.

Application

ELISA, RIA, WB, IHC(P)

Application details

Western blotting: Positive control: human plasma, recommended antibody dilution: 2-5 μg/ml.

Reactivity

Human

Negative species

Rabbit, Mouse, Pig, Cow

Immunogen

Fraction of proteins containing albumin after ammonnium sulphate precipitation and DEAE-chromatography of human serum.

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

HSA, SA

Antigen description

Human serum albumin (65-67 kDa) is the most abundant protein in human blood plasma (produced in the liver). It has a serum half-life of approximately 20 days.

Entrez Gene ID 213

UniProt ID P02768

Western blotting analysis of human serum albumin using mouse monoclonal antibody AL-01 on human plasma and lysate of HeLa cell line (albumin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 5 µg/ml of biotinylated mouse anti-human albumin monoclonal antibody followed by IRDye800-conjugated streptavidin. Albumin was detected at approximately 50-67 kDa.

Product specific references:

Angelisova P, Macku B, Mancal P, Bartek J, Viklicky V: The characteristics of monoclonal antibodies against human albumin. Folia Biol (Praha). 1986;32(5):289-94.
PubMed