Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu Albumin Biotin

Anti-Hu Albumin Biotin

Anti-Hu Albumin Biotin
Regulatory status
RUO
Antigen
Albumin
Clone
AL-01
Format
Biotin
Reactivity
Human
Application
ELISA, RIA, WB, IHC(P)
Variant
0.1 mg
1B-257-C100
In stock
195.00 USD
0.025 mg
1B-257-C025
Delivery 1 week
98.00 USD
Variant
0.1 mg
11-261-C100
In stock
195.00 USD
0.1 mg
11-261-C100
In stock
98.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody AL-01 reacts only with human serum albumin, a 65-67 kDa monomeric protein in human blood plasma; it is produced in liver. No cross-reactivity was observed with other serum proteins.
Application
ELISA, RIA, WB, IHC(P)
Application details
Western blotting: Positive control: human plasma, recommended antibody dilution: 2-5 μg/ml.
Reactivity
Human
Negative species
Rabbit, Mouse, Pig, Cow
Immunogen
Fraction of proteins containing albumin after ammonnium sulphate precipitation and DEAE-chromatography of human serum.
Concentration
1 mg/ml
Preparation
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
HSA, SA
Antigen description
Human serum albumin (65-67 kDa) is the most abundant protein in human blood plasma (produced in the liver). It has a serum half-life of approximately 20 days.
Entrez Gene ID 213
UniProt ID P02768
1B-257_WB
Western blotting analysis of human serum albumin using mouse monoclonal antibody AL-01 on human plasma and lysate of HeLa cell line (albumin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 5 µg/ml of biotinylated mouse anti-human albumin monoclonal antibody followed by IRDye800-conjugated streptavidin. Albumin was detected at approximately 50-67 kDa.

Product specific references:

Angelisova P, Macku B, Mancal P, Bartek J, Viklicky V: The characteristics of monoclonal antibodies against human albumin. Folia Biol (Praha). 1986;32(5):289-94.
PubMed
Variant
0.1 mg
1B-257-C100
In stock
195.00 USD
0.025 mg
1B-257-C025
Delivery 1 week
98.00 USD
Variant
0.1 mg
11-261-C100
In stock
195.00 USD
0.1 mg
11-261-C100
In stock
98.00 USD
Contact distributor
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