Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Hu Albumin Biotin

Anti-Hu Albumin Biotin

Anti-Hu Albumin Biotin
Regulatory status
RUO
Antigen
Albumin
Clone
AL-01
Format
Biotin
Reactivity
Human
Application
ELISA, RIA, WB, IHC(P)
Variant
0.1 mg
1B-257-C100
In stock
0.00 USD
0.025 mg
1B-257-C025
Out of stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD
0.1 mg
11-261-C100
In stock
0.00 USD
Contact distributor
Product details
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References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody AL-01 reacts only with human serum albumin, a 65-67 kDa monomeric protein in human blood plasma; it is produced in liver. No cross-reactivity was observed with other serum proteins.
Application
ELISA, RIA, WB, IHC(P)
Reactivity
Human
Negative species
Non-human primates
Immunogen
Fraction of proteins containing albumin after ammonnium sulphate precipitation and DEAE-chromatography of human serum.
Other names
HSA, SA
Concentration
1 mg/ml
Preparation
The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1B-257_WB
Detection of Human Serum Albumin by Western Blot. Western blot shows human plasma and lysate of HeLa cell line (Albumin non-expressing cell line; negative control). Nitrocellulose membrane was probed with 5 µg/mL of Mouse Anti-Hu Albumin-specific Monoclonal Antibody Biotinylated (clone AL-01) followed by IRDye800-conjugated Streptavidin. A specific bands were detected for Hu Albumin at approximately 50 - 67 kDa. The experiment was conducted under reducing and non-reducing conditions.

Product specific references:

Angelisova P, Macku B, Mancal P, Bartek J, Viklicky V: The characteristics of monoclonal antibodies against human albumin. Folia Biol (Praha). 1986;32(5):289-94.
PubMed
Variant
0.1 mg
1B-257-C100
In stock
0.00 USD
0.025 mg
1B-257-C025
Out of stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD
0.1 mg
11-261-C100
In stock
0.00 USD
Contact distributor
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