Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-C-peptide HRP

Anti-C-peptide HRP

Regulatory status

RUO

Antigen

C-peptide

Clone

C-PEP-01

Format

HRP

Reactivity

Human

Application

ELISA (QC tested)

test1 True

Variant

0.1 mg

1X-247-C100

In stock

225.50 USD

Variant

0.1 mg

1X-247-C100

In stock

225.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody C-PEP-01 reacts specifically with C-peptide, a part of the proinsulin molecule. Proinsulin consists of three parts: C-peptide and two long strands of amino acids (alpha and beta chains; later become linked together to form the insulin molecule). No cross-reactivity with insulin or other peptide hormones or proteins was observed.

Application

ELISA (QC tested)

Reactivity

Human

Immunogen

Human C-peptide conjugated to bovine serum albumin.

Concentration

0.2 mg/ml

Preparation

Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01% thimerosal

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

C-peptide of proinsulin

Antigen description

From every molecule of proinsulin, one molecule of insulin plus one molecule of C-peptide are produced. C-peptide is released into the blood stream in equal amounts to insulin.

ELISA analysis of human C-peptide by mouse monoclonal antibody C-PEP-01. MaxiSorp plate was coated with recombinant human C-peptide, then blocked with BSA, and incubated with dilution series of anti-C-peptide HRP conjugated primary antibody. Colorimetric detection was performed using TMB substrate and absorbance was measured at 450 nm.

Product specific references:

Hilgert I, Stolba P, Kristofova H, Stefanova I, Bendlova B, Lebl M, Horejsi V: A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA. Hybridoma. 1991 Jun;10(3):379-86.
PubMed

Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7.
PubMed