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Exbio
—
Research products
—
Antibodies
—
Serum, plasma, and secreted proteins
—
Anti-Bov Ig Light Chains Purified
Anti-Bov Ig Light Chains Purified
Regulatory status
RUO
Antigen
Ig Light Chains
Clone
IVA285-1
Format
Purified
Reactivity
Sheep, Cow
Application
FC, WB, IHC(F)
Variant
0.1 mg
11-420-C100
In stock
143.00 USD
0.025 mg
11-420-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-420-C100
In stock
143.00 USD
0.025 mg
11-420-C025
Delivery 1 week
71.50 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody IVA285-1 reacts with bovine Ig (light chains). Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
Application
FC, WB, IHC(F)
Application details
Western blotting: Recommended dilution: 0,5-1 μg/ml, overnight at 4°C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12% separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM.
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Sheep, Cow
Immunogen
Fraction of Bovine immunoglobulin.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Immunoglobulin light chains
Western blotting detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and Bovine Serum (right lane) by antibody IVA285-1. This antibody is a suitable tool for in-house quality controls of Fetal Calf Serum (Hybridoma Cell Culture grade).
Separation of bovine Ig Light Chains positive leukocytes (red-filled) from Ig Light Chains negative leukocytes (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine Ig Light Chains (IVA285-1) purified antibody (concentration in sample 3 μg/ml, GAM APC).
Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine Ig Light Chains (IVA285-1) purified antibody (concentration in sample 3 μg/ml, GAM APC).
Product specific references:
Antalikova J, Simon M, Jankovicova J, Horovska L, Dusinsky R, Hluchy S: Monoclonal antibody to the light chain of bovine immunoglobulin. Hybridoma (Larchmt). 2006 Oct;25(5):309-12.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
11-420-C100
In stock
143.00 USD
0.025 mg
11-420-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-420-C100
In stock
143.00 USD
0.025 mg
11-420-C025
Delivery 1 week
71.50 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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