Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Bov Ig Light Chains Purified

Anti-Bov Ig Light Chains Purified

Anti-Bov Ig Light Chains Purified
Regulatory status
RUO
Antigen
Ig Light Chains
Clone
IVA285-1
Format
Purified
Reactivity
Sheep, Cow
Application
FC, WB, IHC(F)
Variant
0.1 mg
11-420-C100
In stock
130.00 USD
0.025 mg
11-420-C025
Out of stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
0.1 mg
11-261-C100
In stock
65.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody IVA285-1 reacts with bovine Ig (light chains). Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
Application
FC, WB, IHC(F)
Application details
Western blotting: Recommended dilution: 0,5-1 μg/ml, overnight at 4°C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12% separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM.
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Sheep, Cow
Immunogen
Fraction of Bovine immunoglobulin.
Other names
Immunoglobulin light chains
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-420 WB
Western blotting detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and Bovine Serum (right lane) by antibody IVA285-1. This antibody is a suitable tool for in-house quality controls of Fetal Calf Serum (Hybridoma Cell Culture grade).

Product specific references:

Antalikova J, Simon M, Jankovicova J, Horovska L, Dusinsky R, Hluchy S: Monoclonal antibody to the light chain of bovine immunoglobulin. Hybridoma (Larchmt). 2006 Oct;25(5):309-12.
PubMed
Variant
0.1 mg
11-420-C100
In stock
130.00 USD
0.025 mg
11-420-C025
Out of stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
0.1 mg
11-261-C100
In stock
65.00 USD
Contact distributor
Datasheet download
SDS download
Bulk requests
Technical support for this product