Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Bov Ig Light Chains Alexa Fluor<sup>®</sup> 488

Anti-Bov Ig Light Chains Alexa Fluor^® 488

Regulatory status

RUO

Antigen

Ig Light Chains

Clone

IVA285-1

Format

Alexa Fluor^® 488

Reactivity

Sheep, Cow

Application

WB, FC (QC tested), IHC-F

Excitation laser

blue (488 nm)

Variant

0.1 mg

A4-420-C100

Ask for delivery term HERE

248.60 USD

Variant

0.1 mg

A4-420-C100

Ask for delivery term HERE

248.60 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody IVA285-1 reacts with bovine Ig (light chains). Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.

Application

WB, FC (QC tested), IHC-F

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Sheep, Cow

Immunogen

Fraction of Bovine immunoglobulin.

Concentration

0.5 mg/ml

Preparation

Purified antibody is conjugated with Alexa Fluor^® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

Immunoglobulin light chains

Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine Ig Light Chain (IVA285-1) Alexa Fluor® 488 antibody (concentration in sample 27 μg/ml).

Flow cytometry multicolor surface staining of bovine lymphocytes stained using anti-bovine Ig Light Chain (IVA285-1) Alexa Fluor® 488 antibody (concentration in sample 27 μg/ml) and anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of bovine Ig Light Chain positive CD19 positive lymphocytes (red-filled) from Ig Light Chain negative CD19 negativce lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine Ig Light Chain (IVA285-1) Alexa Fluor® 488 antibody (concentration in sample 27 μg/ml).

Product specific references:

Antalikova J, Simon M, Jankovicova J, Horovska L, Dusinsky R, Hluchy S: Monoclonal antibody to the light chain of bovine immunoglobulin. Hybridoma (Larchmt). 2006 Oct;25(5):309-12.
PubMed