Anti-alpha-Fetoprotein Purified

Anti-alpha-Fetoprotein Purified
Regulatory status
RUO
Antigen
alpha-Fetoprotein
Clone
AFP-11
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-384-C100
In stock
110.00 USD

0.025 mg
11-384-C025
Delivery 1 week
55.00 USD
Variant
0.1 mg
11-261-C100
In stock
110.00 USD

0.1 mg
11-261-C100
In stock
55.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody AFP-11 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
Application details
ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100).
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: hepatocellular carcinoma; heat mediated antigen retrieval (sodium citrate buffer); mAb incubation 1 hour / RT, detection DAB.
Reactivity
Human
Immunogen
purified alpha-Fetoprotein
Concentration
1 mg/ml
Preparation
Purified by precipitation and chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
AFP, FETA, HPAFP
Antigen description
Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.
Entrez Gene ID 174
UniProt ID P02771
11-384_IHC
Immunohistochemistry staining of hepatocellular carcinoma (fetal liver; paraffin-embedded sections) with anti-human alpha-Fetoprotein (AFP-11).

Product specific references:

Stefanova I, Horejsi V, Kristofova H, Angelisova P, Zizkovsky V, Hilgert I: Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes. J Immunol Methods. 1988 Jun 28;111(1):67-73.
PubMed
Variant
0.1 mg
11-384-C100
In stock
110.00 USD

0.025 mg
11-384-C025
Delivery 1 week
55.00 USD
Variant
0.1 mg
11-261-C100
In stock
110.00 USD

0.1 mg
11-261-C100
In stock
55.00 USD