Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-alpha-Fetoprotein Purified

Anti-alpha-Fetoprotein Purified

Regulatory status

RUO

Antigen

alpha-Fetoprotein

Clone

AFP-11

Format

Purified

Reactivity

Human

Application

ELISA, RIA, WB, IHC(P)

Variant

0.1 mg

11-384-C100

In stock

110.00 USD

0.025 mg

11-384-C025

Delivery 1 week

55.00 USD

Variant

0.1 mg

11-384-C100

In stock

110.00 USD

0.025 mg

11-384-C025

Delivery 1 week

55.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody AFP-11 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.

Application

ELISA, RIA, WB, IHC(P)

Application details

ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100).
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: hepatocellular carcinoma; heat mediated antigen retrieval (sodium citrate buffer); mAb incubation 1 hour / RT, detection DAB.
Western blotting: Recommended dilution: 2 μg/ml, non-reducing conditions.

Reactivity

Human

Immunogen

purified alpha-Fetoprotein

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

AFP, FETA, HPAFP

Antigen description

Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.

Entrez Gene ID 174

UniProt ID P02771

Immunohistochemistry staining of hepatocellular carcinoma (fetal liver; paraffin-embedded sections) with anti-human alpha-Fetoprotein (AFP-11).

Western blotting analysis of human alpha-fetoprotein using mouse monoclonal antibody AFP-11 on lysates of HepG2 cell line and Jurkat cell line (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-alpha-fetoprotein followed by IRDye800-conjugated anti-mouse secondary antibody. Alpha-fetoprotein was detected at approximately 60 kDa.

Product specific references:

Stefanova I, Horejsi V, Kristofova H, Angelisova P, Zizkovsky V, Hilgert I: Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes. J Immunol Methods. 1988 Jun 28;111(1):67-73.
PubMed